Single-cell detection using a thin film transistor photosensor with micro-partitions

Tsuyoshi Tanaka, Yoshihiko Sunaga, Keiichi Hatakeyama, Tadashi Matsunaga

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

A thin film transistor (TFT) photosensor was applied to single-cell detection by identifying cell surface molecules based on chemiluminescence. Micro-partitions were directly fabricated on the TFT photosensor surface by photolithography. The surface of each pixel was surrounded by 25 μm-height partitions, forming areas of approximately 30 μm × 30 μm for cell entrapment and photosensing. Visualization of individual JM cells, stained with mouse anti-human CD8 IgG1 primary antibody and Horseradish peroxidase (HRP)-labeled anti-mouse IgG1 secondary antibody, as bright-pixels was successfully achieved using the micro-partitioned TFT photosensor integrated into a microfluidic chamber. Furthermore, real-time monitoring of HRP-labeled JM cells was also accomplished. The fabrication of micro-partitions on the surface of the TFT photosensor allows highly efficient single-cell entrapment and chemiluminescence-based detection of JM cells. This is the first report of single-cell entrapment and subsequent signal detection on the photosensing area of individual pixels of TFT photosensor. This system will allow high-throughput and real-time analysis of more than 104 cells with minimum optical system requirements.

Original languageEnglish
Pages (from-to)3348-3354
Number of pages7
JournalLab on a Chip
Volume10
Issue number24
DOIs
Publication statusPublished - 2010 Dec 21
Externally publishedYes

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Biochemistry
  • Biomedical Engineering

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