Single-cell observation of phagocytosis by human blood dendritic cells

Hiroshi Ishimoto, Katsunori Yanagihara, Nobuko Araki, Hiroshi Mukae, Noriho Sakamoto, Koichi Izumikawa, Masafumi Seki, Yoshitsugu Miyazaki, Yoichi Hirakata, Yohei Mizuta, Kenji Yasuda, Shigeru Kohno

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Time-lapse video microscopic observation is useful for analysis of cell biology, especially in rapid response of immune cells. Dendritic cells (DCs) have multiple functions in the immune system, and DCs in peripheral blood play an especially important role at the front line of infection. We have developed a time-lapse video microscopic method for the evaluation of single myeloid DCs (MDC-1) from human peripheral blood. MDC-1 displayed generalized plasma membrane ruffling and phagocytosis of Pseudomonas aeruginosa. The morphological changes of MDC-1 increased following stimulation with P. aeruginosa but not after stimulation with supernatant from a P. aeruginosa culture. The activation of these morphological changes in MDC-1 could be quantitatively analyzed using the time-lapse video microscopy. This novel system may be useful for the evaluation of rapid response with human immune cells against bacterial infection.

Original languageEnglish
Pages (from-to)294-297
Number of pages4
JournalJapanese Journal of Infectious Diseases
Volume61
Issue number4
Publication statusPublished - 2008 Aug 11
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

Fingerprint Dive into the research topics of 'Single-cell observation of phagocytosis by human blood dendritic cells'. Together they form a unique fingerprint.

  • Cite this

    Ishimoto, H., Yanagihara, K., Araki, N., Mukae, H., Sakamoto, N., Izumikawa, K., Seki, M., Miyazaki, Y., Hirakata, Y., Mizuta, Y., Yasuda, K., & Kohno, S. (2008). Single-cell observation of phagocytosis by human blood dendritic cells. Japanese Journal of Infectious Diseases, 61(4), 294-297.