Single-cell real-time imaging of flow-induced hemolysis using high-speed microfluidic technology

Takanobu Yagi, S. Wakasa, N. Tokunaga, Y. Akimoto, Mitsuo Umezu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Understanding the mechanism of flow-induced blood cell damage, such as hemolysis and platelet activation, plays an important role for arterial diseases and artificial organs. This study for the first time demonstrates the visualization of flow-induced hemolysis in a single-cell real-time manner using high-speed microfluidic technology. Impinging microjets with a velocity of 1.5 m/s order at a nozzle exit were made in the Y- and T-shaped microchannel. The curved (r=10μm) and flat collision surface were compared. Porcine fresh erythrocytes were suspended in PBS at Ht=0.5%. Results showed that membrane failure was only observed in the Y-junction. These erythrocytes were initially elongated at the far region, and then longitudinally compressed in the near wall region due to the sharp adverse pressure gradient, whereas those in the T-junction released the membrane tension as the pressure difference per erythrocyte diminished. In the simulation of energy balance, it was found that the dominant force for the longitudinal compression was the pressure difference per erythrocyte. Such erythrocytes showed the sudden drop of elastic modulus, suggesting that the elongated spectrin network of erythrocyte was fragile for the compressive force and immediately broken by the impact force.

Original languageEnglish
Title of host publicationIFMBE Proceedings
Pages2337-2340
Number of pages4
Volume25
Edition4
DOIs
Publication statusPublished - 2009
EventWorld Congress on Medical Physics and Biomedical Engineering: Image Processing, Biosignal Processing, Modelling and Simulation, Biomechanics - Munich
Duration: 2009 Sep 72009 Sep 12

Other

OtherWorld Congress on Medical Physics and Biomedical Engineering: Image Processing, Biosignal Processing, Modelling and Simulation, Biomechanics
CityMunich
Period09/9/709/9/12

Fingerprint

Microfluidics
Artificial organs
Membranes
Imaging techniques
Spectrin
Platelets
Microchannels
Energy balance
Pressure gradient
Nozzles
Blood
Visualization
Elastic moduli
Chemical activation
Cells

Keywords

  • Hemolysis
  • Micro
  • Red blood cell
  • Spectrin

ASJC Scopus subject areas

  • Biomedical Engineering
  • Bioengineering

Cite this

Single-cell real-time imaging of flow-induced hemolysis using high-speed microfluidic technology. / Yagi, Takanobu; Wakasa, S.; Tokunaga, N.; Akimoto, Y.; Umezu, Mitsuo.

IFMBE Proceedings. Vol. 25 4. ed. 2009. p. 2337-2340.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Yagi, T, Wakasa, S, Tokunaga, N, Akimoto, Y & Umezu, M 2009, Single-cell real-time imaging of flow-induced hemolysis using high-speed microfluidic technology. in IFMBE Proceedings. 4 edn, vol. 25, pp. 2337-2340, World Congress on Medical Physics and Biomedical Engineering: Image Processing, Biosignal Processing, Modelling and Simulation, Biomechanics, Munich, 09/9/7. https://doi.org/10.1007/978-3-642-03882-2-621
Yagi, Takanobu ; Wakasa, S. ; Tokunaga, N. ; Akimoto, Y. ; Umezu, Mitsuo. / Single-cell real-time imaging of flow-induced hemolysis using high-speed microfluidic technology. IFMBE Proceedings. Vol. 25 4. ed. 2009. pp. 2337-2340
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