Single cell real time secretion assay using amorphous fluoropolymer microwell array

Y. Shirasaki, A. Nakahara, N. Shimura, M. Yamagishi, Jun Mizuno, O. Ohara, Shuichi Shoji

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

Real time assay of protein secretion from living single cells was realized by coupling total internal reflection fluorescence (TIRF) microscopy observation and an optically optimized microwell array device. This system was based on a sandwich immunosorbent assay. Individual cells were cultured on an inverted microscope in microwell arrays fabricated of an amorphous fluoropolymer, CYTOP, to be monitored by TIRF imaging. CYTOP have no interference for the TIRF imaging because the refractive index of CYTOP is almost same as that of water. Using this system, we could succeed in real time secretion assay of lipopolysaccharide stimulated mast cells and the secretion time course of IL-1β from a single MC/9 cell was obtained successfully.

Original languageEnglish
Title of host publication2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11
Pages755-758
Number of pages4
DOIs
Publication statusPublished - 2011
Event2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11 - Beijing
Duration: 2011 Jun 52011 Jun 9

Other

Other2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11
CityBeijing
Period11/6/511/6/9

Fingerprint

Fluorine containing polymers
Assays
Fluorescence
Imaging techniques
Wave interference
Fluorescence microscopy
Refractive index
Microscopes
Proteins
Water

Keywords

  • CYTOP
  • secretion assay
  • single cell
  • TIRF

ASJC Scopus subject areas

  • Hardware and Architecture
  • Electrical and Electronic Engineering

Cite this

Shirasaki, Y., Nakahara, A., Shimura, N., Yamagishi, M., Mizuno, J., Ohara, O., & Shoji, S. (2011). Single cell real time secretion assay using amorphous fluoropolymer microwell array. In 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11 (pp. 755-758). [5969330] https://doi.org/10.1109/TRANSDUCERS.2011.5969330

Single cell real time secretion assay using amorphous fluoropolymer microwell array. / Shirasaki, Y.; Nakahara, A.; Shimura, N.; Yamagishi, M.; Mizuno, Jun; Ohara, O.; Shoji, Shuichi.

2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11. 2011. p. 755-758 5969330.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Shirasaki, Y, Nakahara, A, Shimura, N, Yamagishi, M, Mizuno, J, Ohara, O & Shoji, S 2011, Single cell real time secretion assay using amorphous fluoropolymer microwell array. in 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11., 5969330, pp. 755-758, 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11, Beijing, 11/6/5. https://doi.org/10.1109/TRANSDUCERS.2011.5969330
Shirasaki Y, Nakahara A, Shimura N, Yamagishi M, Mizuno J, Ohara O et al. Single cell real time secretion assay using amorphous fluoropolymer microwell array. In 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11. 2011. p. 755-758. 5969330 https://doi.org/10.1109/TRANSDUCERS.2011.5969330
Shirasaki, Y. ; Nakahara, A. ; Shimura, N. ; Yamagishi, M. ; Mizuno, Jun ; Ohara, O. ; Shoji, Shuichi. / Single cell real time secretion assay using amorphous fluoropolymer microwell array. 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11. 2011. pp. 755-758
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