Single cell real time secretion assay using amorphous fluoropolymer microwell array

Y. Shirasaki; A. Nakahara; N. Shimura; M. Yamagishi; J. Mizuno; O. Ohara; S. Shoji

doi:10.1109/TRANSDUCERS.2011.5969330

Single cell real time secretion assay using amorphous fluoropolymer microwell array

Y. Shirasaki^*, A. Nakahara, N. Shimura, M. Yamagishi, J. Mizuno, O. Ohara, S. Shoji

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

3 Citations (Scopus)

Abstract

Real time assay of protein secretion from living single cells was realized by coupling total internal reflection fluorescence (TIRF) microscopy observation and an optically optimized microwell array device. This system was based on a sandwich immunosorbent assay. Individual cells were cultured on an inverted microscope in microwell arrays fabricated of an amorphous fluoropolymer, CYTOP, to be monitored by TIRF imaging. CYTOP have no interference for the TIRF imaging because the refractive index of CYTOP is almost same as that of water. Using this system, we could succeed in real time secretion assay of lipopolysaccharide stimulated mast cells and the secretion time course of IL-1β from a single MC/9 cell was obtained successfully.

Original language	English
Title of host publication	2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11
Pages	755-758
Number of pages	4
DOIs	https://doi.org/10.1109/TRANSDUCERS.2011.5969330
Publication status	Published - 2011
Event	2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11 - Beijing, China Duration: 2011 Jun 5 → 2011 Jun 9

Publication series

Name	2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11

Conference

Conference	2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11
Country/Territory	China
City	Beijing
Period	11/6/5 → 11/6/9

Keywords

CYTOP
TIRF
secretion assay
single cell

ASJC Scopus subject areas

Hardware and Architecture
Electrical and Electronic Engineering

Access to Document

10.1109/TRANSDUCERS.2011.5969330

Cite this

Shirasaki, Y., Nakahara, A., Shimura, N., Yamagishi, M., Mizuno, J., Ohara, O., & Shoji, S. (2011). Single cell real time secretion assay using amorphous fluoropolymer microwell array. In 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11 (pp. 755-758). [5969330] (2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11). https://doi.org/10.1109/TRANSDUCERS.2011.5969330

Single cell real time secretion assay using amorphous fluoropolymer microwell array. / Shirasaki, Y.; Nakahara, A.; Shimura, N. et al.

2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11. 2011. p. 755-758 5969330 (2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Shirasaki, Y, Nakahara, A, Shimura, N, Yamagishi, M, Mizuno, J, Ohara, O & Shoji, S 2011, Single cell real time secretion assay using amorphous fluoropolymer microwell array. in 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11., 5969330, 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11, pp. 755-758, 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11, Beijing, China, 11/6/5. https://doi.org/10.1109/TRANSDUCERS.2011.5969330

Shirasaki Y, Nakahara A, Shimura N, Yamagishi M, Mizuno J, Ohara O et al. Single cell real time secretion assay using amorphous fluoropolymer microwell array. In 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11. 2011. p. 755-758. 5969330. (2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11). doi: 10.1109/TRANSDUCERS.2011.5969330

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title = "Single cell real time secretion assay using amorphous fluoropolymer microwell array",

abstract = "Real time assay of protein secretion from living single cells was realized by coupling total internal reflection fluorescence (TIRF) microscopy observation and an optically optimized microwell array device. This system was based on a sandwich immunosorbent assay. Individual cells were cultured on an inverted microscope in microwell arrays fabricated of an amorphous fluoropolymer, CYTOP, to be monitored by TIRF imaging. CYTOP have no interference for the TIRF imaging because the refractive index of CYTOP is almost same as that of water. Using this system, we could succeed in real time secretion assay of lipopolysaccharide stimulated mast cells and the secretion time course of IL-1β from a single MC/9 cell was obtained successfully.",

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author = "Y. Shirasaki and A. Nakahara and N. Shimura and M. Yamagishi and J. Mizuno and O. Ohara and S. Shoji",

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doi = "10.1109/TRANSDUCERS.2011.5969330",

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AU - Shirasaki, Y.

AU - Nakahara, A.

AU - Shimura, N.

AU - Yamagishi, M.

AU - Mizuno, J.

AU - Ohara, O.

AU - Shoji, S.

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N2 - Real time assay of protein secretion from living single cells was realized by coupling total internal reflection fluorescence (TIRF) microscopy observation and an optically optimized microwell array device. This system was based on a sandwich immunosorbent assay. Individual cells were cultured on an inverted microscope in microwell arrays fabricated of an amorphous fluoropolymer, CYTOP, to be monitored by TIRF imaging. CYTOP have no interference for the TIRF imaging because the refractive index of CYTOP is almost same as that of water. Using this system, we could succeed in real time secretion assay of lipopolysaccharide stimulated mast cells and the secretion time course of IL-1β from a single MC/9 cell was obtained successfully.

AB - Real time assay of protein secretion from living single cells was realized by coupling total internal reflection fluorescence (TIRF) microscopy observation and an optically optimized microwell array device. This system was based on a sandwich immunosorbent assay. Individual cells were cultured on an inverted microscope in microwell arrays fabricated of an amorphous fluoropolymer, CYTOP, to be monitored by TIRF imaging. CYTOP have no interference for the TIRF imaging because the refractive index of CYTOP is almost same as that of water. Using this system, we could succeed in real time secretion assay of lipopolysaccharide stimulated mast cells and the secretion time course of IL-1β from a single MC/9 cell was obtained successfully.

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ER -

Single cell real time secretion assay using amorphous fluoropolymer microwell array

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