TY - JOUR
T1 - Single nucleotide mismatch analysis using oligonucleotide probes synthesized on bacterial magnetic particle
AU - Ota, Hiroyuki
AU - Arakaki, Atsushi
AU - Tanaka, Tsuyoshi
AU - Takeyama, Haruko
AU - Matsunaga, Tadashi
N1 - Funding Information:
This work was funded in part by a Grant-in-Aid for Specially Promoted Research (2), No. 13002005 from the Scientific Research for the Ministry of Education, Culture, Sports, Science and Technology of Japan and as part of the 21st Century Center of Excellence (COE) program of “Future Nano-Materials” research and education project through Tokyo University of Agriculture and Technology.
PY - 2003/7
Y1 - 2003/7
N2 - An approach to analyze mismatches using short and specific oligonucleotide probes directly synthesized on bacterial magnetic particles (BMPs) by phosphoramidite methods was exploited. Approximately 126 molecules of 4-mer oligonucleotides/particle were synthesized on BMPs with high reaction efficiencies. Hybridization between FITC-labeled oligonucleotides and chemically synthesized oligonucleotides on BMPs was performed. Perfect matched and mismatched hybridizations were successfully discriminated by using the oligonucleotide probes on BMPs.
AB - An approach to analyze mismatches using short and specific oligonucleotide probes directly synthesized on bacterial magnetic particles (BMPs) by phosphoramidite methods was exploited. Approximately 126 molecules of 4-mer oligonucleotides/particle were synthesized on BMPs with high reaction efficiencies. Hybridization between FITC-labeled oligonucleotides and chemically synthesized oligonucleotides on BMPs was performed. Perfect matched and mismatched hybridizations were successfully discriminated by using the oligonucleotide probes on BMPs.
KW - Bacterial magnetic particles
KW - Hybridization
KW - Mismatch discrimination
KW - Oligonucleotide synthesis
UR - http://www.scopus.com/inward/record.url?scp=0042567288&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0042567288&partnerID=8YFLogxK
U2 - 10.1016/S1389-0344(03)00036-4
DO - 10.1016/S1389-0344(03)00036-4
M3 - Article
C2 - 12919813
AN - SCOPUS:0042567288
SN - 1871-6784
VL - 20
SP - 305
EP - 309
JO - Biomolecular Engineering
JF - Biomolecular Engineering
IS - 4-6
ER -