Single nucleotide mismatch analysis using oligonucleotide probes synthesized on bacterial magnetic particle

Hiroyuki Ota, Atsushi Arakaki, Tsuyoshi Tanaka, Haruko Takeyama, Tadashi Matsunaga

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

An approach to analyze mismatches using short and specific oligonucleotide probes directly synthesized on bacterial magnetic particles (BMPs) by phosphoramidite methods was exploited. Approximately 126 molecules of 4-mer oligonucleotides/particle were synthesized on BMPs with high reaction efficiencies. Hybridization between FITC-labeled oligonucleotides and chemically synthesized oligonucleotides on BMPs was performed. Perfect matched and mismatched hybridizations were successfully discriminated by using the oligonucleotide probes on BMPs.

Original languageEnglish
Pages (from-to)305-309
Number of pages5
JournalBiomolecular Engineering
Volume20
Issue number4-6
DOIs
Publication statusPublished - 2003 Jul
Externally publishedYes

Keywords

  • Bacterial magnetic particles
  • Hybridization
  • Mismatch discrimination
  • Oligonucleotide synthesis

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Molecular Biology

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