Single-stage autotrophic nitrogen-removal process using a composite matrix immobilizing nitrifying and sulfur-denitrifying bacteria

Y. Aoi, Y. Shiramasa, E. Kakimoto, Satoshi Tsuneda, A. Hirata, T. Nagamune

    Research output: Contribution to journalArticle

    11 Citations (Scopus)

    Abstract

    We developed a novel single-stage autotrophic nitrogen-removal process comprised of two composite immobilized biomass layers-one of nitrifying bacteria and one of sulfur-denitrifying bacteria and elemental sulfur-in a Fe-Ni fibrous slag matrix. Nitrification and consumption of dissolved oxygen occurred in the outer part and sulfur denitrification in the anoxic inner part of the composite matrix, thus realizing autotrophic nitrogen removal in a single reactor. The complete conversion of ammonia into N2 in a single reactor was demonstrated in both batch-mode incubation and continuous-feed operation. The spatial profiles of the ammonia-oxidizing bacteria and denitrifying bacteria were evaluated by real-time PCR, targeting their functional genes, and stratification of these two types was observed in the matrix after several months of incubation. This process does not require any specific reactor type or conditions and thus has the potential to be applied to many different wastewater treatment processes due to its simplicity in both operation and construction.

    Original languageEnglish
    Pages (from-to)124-130
    Number of pages7
    JournalApplied Microbiology and Biotechnology
    Volume68
    Issue number1
    DOIs
    Publication statusPublished - 2005 Aug

    Fingerprint

    Nitrogen removal
    Sulfur
    Bacteria
    Nitrogen
    Composite materials
    Ammonia
    Nitrification
    Denitrification
    Dissolved oxygen
    Waste Water
    Oxygen Consumption
    Wastewater treatment
    Biomass
    Slags
    Real-Time Polymerase Chain Reaction
    Genes

    ASJC Scopus subject areas

    • Biotechnology
    • Microbiology
    • Bioengineering
    • Microbiology (medical)

    Cite this

    Single-stage autotrophic nitrogen-removal process using a composite matrix immobilizing nitrifying and sulfur-denitrifying bacteria. / Aoi, Y.; Shiramasa, Y.; Kakimoto, E.; Tsuneda, Satoshi; Hirata, A.; Nagamune, T.

    In: Applied Microbiology and Biotechnology, Vol. 68, No. 1, 08.2005, p. 124-130.

    Research output: Contribution to journalArticle

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