Site-selective immobilization of streptavidin on enzymatically biotinylated bacterial magnetic particles

Yoshiaki Maeda, Tomoko Yoshino, Haruko Takeyama, Masaaki Takahashi, Harumi Ginya, Junko Asahina, Hideji Tajima, Tadashi Matsunaga

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Biotinylated magnetic nanoparticles were constructed by displaying biotin acceptor peptide (BAP) on the surfaces of bacterial magnetic particles (BacMPs) synthesized by Magnetospirillum niagneticum AMB-1. Both BAP and green fluorescent protein (GFP) were fused to Mmsl3 that was isolated from BacMP membranes. The localization of the fusion protein, BAP-Mmsl3-GFP, was confirmed by fluorescence analysis. BacMPs that expressed B AP-Mms 13-GFP (BAP/GFP-BacMPs) were extracted from bacterial cells and incubated with biotin and Escherichia coli biotin ligase. The in vitro biotinylation of BAP/GFP-BacMPs was confirmed using alkaline phosphatase (ALP)-labeled streptavidin. The conjugation system developed in this study provides a method for producing biotin- or streptavidin-labeled magnetic nanoparticles without the use of a crosslinker reagent. Various functional materials can be immobilized site-selectively onto these uniquely designed BacMPs. By combining this site-selective biotinylation technology and protein display methodology, increasingly innovative and attractive magnetic nano-materials can be constructed.

Original languageEnglish
Title of host publicationMaterials Research Society Symposium Proceedings
Pages65-70
Number of pages6
Volume1094
Publication statusPublished - 2008
Externally publishedYes
Event2008 MRS Spring Meeting - San Francisco, CA
Duration: 2008 Mar 242008 Mar 28

Other

Other2008 MRS Spring Meeting
CitySan Francisco, CA
Period08/3/2408/3/28

Fingerprint

biotin
Streptavidin
Biotin
immobilization
Green Fluorescent Proteins
proteins
Peptides
peptides
Proteins
Nanoparticles
nanoparticles
phosphatases
Functional materials
Phosphatases
Ligases
Escherichia
conjugation
Escherichia coli
Alkaline Phosphatase
reagents

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering
  • Mechanics of Materials

Cite this

Maeda, Y., Yoshino, T., Takeyama, H., Takahashi, M., Ginya, H., Asahina, J., ... Matsunaga, T. (2008). Site-selective immobilization of streptavidin on enzymatically biotinylated bacterial magnetic particles. In Materials Research Society Symposium Proceedings (Vol. 1094, pp. 65-70)

Site-selective immobilization of streptavidin on enzymatically biotinylated bacterial magnetic particles. / Maeda, Yoshiaki; Yoshino, Tomoko; Takeyama, Haruko; Takahashi, Masaaki; Ginya, Harumi; Asahina, Junko; Tajima, Hideji; Matsunaga, Tadashi.

Materials Research Society Symposium Proceedings. Vol. 1094 2008. p. 65-70.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Maeda, Y, Yoshino, T, Takeyama, H, Takahashi, M, Ginya, H, Asahina, J, Tajima, H & Matsunaga, T 2008, Site-selective immobilization of streptavidin on enzymatically biotinylated bacterial magnetic particles. in Materials Research Society Symposium Proceedings. vol. 1094, pp. 65-70, 2008 MRS Spring Meeting, San Francisco, CA, 08/3/24.
Maeda Y, Yoshino T, Takeyama H, Takahashi M, Ginya H, Asahina J et al. Site-selective immobilization of streptavidin on enzymatically biotinylated bacterial magnetic particles. In Materials Research Society Symposium Proceedings. Vol. 1094. 2008. p. 65-70
Maeda, Yoshiaki ; Yoshino, Tomoko ; Takeyama, Haruko ; Takahashi, Masaaki ; Ginya, Harumi ; Asahina, Junko ; Tajima, Hideji ; Matsunaga, Tadashi. / Site-selective immobilization of streptavidin on enzymatically biotinylated bacterial magnetic particles. Materials Research Society Symposium Proceedings. Vol. 1094 2008. pp. 65-70
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