Site-selective immobilization of streptavidin on enzymatically biotinylated bacterial magnetic particles

Yoshiaki Maeda, Tomoko Yoshino, Haruko Takeyama, Masaaki Takahashi, Harumi Ginya, Junko Asahina, Hideji Tajima, Tadashi Matsunaga

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Biotinylated magnetic nanoparticles were constructed by displaying biotin acceptor peptide (BAP) on the surfaces of bacterial magnetic particles (BacMPs) synthesized by Magnetospirillum niagneticum AMB-1. Both BAP and green fluorescent protein (GFP) were fused to Mmsl3 that was isolated from BacMP membranes. The localization of the fusion protein, BAP-Mmsl3-GFP, was confirmed by fluorescence analysis. BacMPs that expressed B AP-Mms 13-GFP (BAP/GFP-BacMPs) were extracted from bacterial cells and incubated with biotin and Escherichia coli biotin ligase. The in vitro biotinylation of BAP/GFP-BacMPs was confirmed using alkaline phosphatase (ALP)-labeled streptavidin. The conjugation system developed in this study provides a method for producing biotin- or streptavidin-labeled magnetic nanoparticles without the use of a crosslinker reagent. Various functional materials can be immobilized site-selectively onto these uniquely designed BacMPs. By combining this site-selective biotinylation technology and protein display methodology, increasingly innovative and attractive magnetic nano-materials can be constructed.

Original languageEnglish
Title of host publicationFrom Biological Materials to Biomimetic Material Synthesis
Pages65-70
Number of pages6
Publication statusPublished - 2008 Dec 1
Externally publishedYes
Event2008 MRS Spring Meeting - San Francisco, CA, United States
Duration: 2008 Mar 242008 Mar 28

Publication series

NameMaterials Research Society Symposium Proceedings
Volume1094
ISSN (Print)0272-9172

Conference

Conference2008 MRS Spring Meeting
CountryUnited States
CitySan Francisco, CA
Period08/3/2408/3/28

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

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  • Cite this

    Maeda, Y., Yoshino, T., Takeyama, H., Takahashi, M., Ginya, H., Asahina, J., Tajima, H., & Matsunaga, T. (2008). Site-selective immobilization of streptavidin on enzymatically biotinylated bacterial magnetic particles. In From Biological Materials to Biomimetic Material Synthesis (pp. 65-70). (Materials Research Society Symposium Proceedings; Vol. 1094).