TY - JOUR
T1 - Site-specific gene expression analysis using an automated tissue micro-dissection punching system
AU - Yoda, Takuya
AU - Hosokawa, Masahito
AU - Takahashi, Kiyofumi
AU - Sakanashi, Chikako
AU - Takeyama, Haruko
AU - Kambara, Hideki
N1 - Funding Information:
We thank Central Research Laboratory of Hitachi Ltd. (especially Dr. H. Matsunaga) for teaching single-cell RNA-seq, offering prototype punching instrument, and the dispenser. This research was supported by the Platform Project for Supporting in Drug Discovery and Life Science Research (Platform for Drug Discovery, Informatics, and Structural Life Science) from Japan Agency for Medical Research and Development (AMED) and also by Hitachi and Waseda University collaboration program. M.H. was supported by JST-PRESTO Grant Number JPMJPR15FA. We thank Human Genome Center (the Univ. of Tokyo) for providing the supercomputing resource (http://sc.hgc.jp/shirokane.html).
Publisher Copyright:
© The Author(s) 2017.
PY - 2017/12/1
Y1 - 2017/12/1
N2 - Site-specific gene expression analyses are important for understanding tissue functions. Despite rapid developments in DNA-related technologies, the site-specific analysis of whole genome expression for a tissue remains challenging. Thus, a new tool is required for capturing multiple tissue micro-dissections or single cells while retaining the positional information. Here, we describe the development of such a system, which can pick up micro-dissections by punching a tissue repeatedly in a very short period, e.g., 5 s/sampling cycle. A photo of the punched tissue provides information on the dissected positions, allowing site-specific gene expression analysis. We demonstrate the site-specific analysis of a frozen tissue slice of mouse brain by analyzing many micro-dissections produced from the tissue at a 300-μm pitch. The site-specific analysis provided new insights into the gene expression profiles in a tissue and on tissue functions. The analysis of site-specific whole genome expression may therefore, open new avenues in life science.
AB - Site-specific gene expression analyses are important for understanding tissue functions. Despite rapid developments in DNA-related technologies, the site-specific analysis of whole genome expression for a tissue remains challenging. Thus, a new tool is required for capturing multiple tissue micro-dissections or single cells while retaining the positional information. Here, we describe the development of such a system, which can pick up micro-dissections by punching a tissue repeatedly in a very short period, e.g., 5 s/sampling cycle. A photo of the punched tissue provides information on the dissected positions, allowing site-specific gene expression analysis. We demonstrate the site-specific analysis of a frozen tissue slice of mouse brain by analyzing many micro-dissections produced from the tissue at a 300-μm pitch. The site-specific analysis provided new insights into the gene expression profiles in a tissue and on tissue functions. The analysis of site-specific whole genome expression may therefore, open new avenues in life science.
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U2 - 10.1038/s41598-017-04616-6
DO - 10.1038/s41598-017-04616-6
M3 - Article
C2 - 28659603
AN - SCOPUS:85021680986
SN - 2045-2322
VL - 7
JO - Scientific Reports
JF - Scientific Reports
IS - 1
M1 - 4325
ER -