Sixty new STSs (sequence-tagged sites) of human chromosome 21

Hiroshi Tanahashi, Takashi Ito, Masahira Hattori, Miyuki Ohira, Misao Ohki, Kosuke Tashiro, Yoshiyuki Sakaki

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

From human chromosome 21-specific libraries, 22 SfiI linking clones and 38 P1 clones were isolated and regionally mapped on the chromosome. The terminal sequences of these clones were determined and pairs of PCR primers were generated which could specifically amplify the sequenced regions. These sequence-tagged sites (STSs) should be useful for constructing a high resolution map of human chromosome 21.

Original languageEnglish
Pages (from-to)85-89
Number of pages5
JournalDNA Research
Volume1
Issue number2
Publication statusPublished - 1994
Externally publishedYes

Fingerprint

Sequence Tagged Sites
Chromosomes, Human, Pair 21
Human Chromosomes
Clone Cells
Libraries
Chromosomes
Polymerase Chain Reaction

Keywords

  • Human chromosome restriction enzyme SfiI
  • Linking clone
  • P1 clone, high-resolution physical map
  • PCR

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

Cite this

Tanahashi, H., Ito, T., Hattori, M., Ohira, M., Ohki, M., Tashiro, K., & Sakaki, Y. (1994). Sixty new STSs (sequence-tagged sites) of human chromosome 21. DNA Research, 1(2), 85-89.

Sixty new STSs (sequence-tagged sites) of human chromosome 21. / Tanahashi, Hiroshi; Ito, Takashi; Hattori, Masahira; Ohira, Miyuki; Ohki, Misao; Tashiro, Kosuke; Sakaki, Yoshiyuki.

In: DNA Research, Vol. 1, No. 2, 1994, p. 85-89.

Research output: Contribution to journalArticle

Tanahashi, H, Ito, T, Hattori, M, Ohira, M, Ohki, M, Tashiro, K & Sakaki, Y 1994, 'Sixty new STSs (sequence-tagged sites) of human chromosome 21', DNA Research, vol. 1, no. 2, pp. 85-89.
Tanahashi H, Ito T, Hattori M, Ohira M, Ohki M, Tashiro K et al. Sixty new STSs (sequence-tagged sites) of human chromosome 21. DNA Research. 1994;1(2):85-89.
Tanahashi, Hiroshi ; Ito, Takashi ; Hattori, Masahira ; Ohira, Miyuki ; Ohki, Misao ; Tashiro, Kosuke ; Sakaki, Yoshiyuki. / Sixty new STSs (sequence-tagged sites) of human chromosome 21. In: DNA Research. 1994 ; Vol. 1, No. 2. pp. 85-89.
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