Size-dependent regulation of synchronized activity in living neuronal networks

Hideaki Yamamoto, Shigeru Kubota, Yudai Chida, Mayu Morita, Satoshi Moriya, Hisanao Akima, Shigeo Sato, Ayumi Hirano-Iwata, Takashi Tanii, Michio Niwano

    Research output: Contribution to journalArticle

    10 Citations (Scopus)

    Abstract

    We study the effect of network size on synchronized activity in living neuronal networks. Dissociated cortical neurons form synaptic connections in culture and generate synchronized spontaneous activity within 10 days in vitro. Using micropatterned surfaces to extrinsically control the size of neuronal networks, we show that synchronized activity can emerge in a network as small as 12 cells. Furthermore, a detailed comparison of small (∼20 cells), medium (∼100 cells), and large (∼400 cells) networks reveal that synchronized activity becomes destabilized in the small networks. A computational modeling of neural activity is then employed to explore the underlying mechanism responsible for the size effect. We find that the generation and maintenance of the synchronized activity can be minimally described by: (1) the stochastic firing of each neuron in the network, (2) enhancement in the network activity in a positive feedback loop of excitatory synapses, and (3) Ca-dependent suppression of bursting activity. The model further shows that the decrease in total synaptic input to a neuron that drives the positive feedback amplification of correlated activity is a key factor underlying the destabilization of synchrony in smaller networks. Spontaneous neural activity plays a critical role in cortical information processing, and our work constructively clarifies an aspect of the structural basis behind this.

    Original languageEnglish
    Article number012407
    JournalPhysical Review E - Statistical, Nonlinear, and Soft Matter Physics
    Volume94
    Issue number1
    DOIs
    Publication statusPublished - 2016 Jul 13

    Fingerprint

    Neuronal Network
    Dependent
    neurons
    Neuron
    positive feedback
    Positive Feedback
    Cell
    cells
    synapses
    Bursting
    Synchrony
    Synapse
    Computational Modeling
    Size Effect
    destabilization
    Feedback Loop
    Information Processing
    Amplification
    maintenance
    Maintenance

    ASJC Scopus subject areas

    • Statistical and Nonlinear Physics
    • Statistics and Probability
    • Condensed Matter Physics

    Cite this

    Size-dependent regulation of synchronized activity in living neuronal networks. / Yamamoto, Hideaki; Kubota, Shigeru; Chida, Yudai; Morita, Mayu; Moriya, Satoshi; Akima, Hisanao; Sato, Shigeo; Hirano-Iwata, Ayumi; Tanii, Takashi; Niwano, Michio.

    In: Physical Review E - Statistical, Nonlinear, and Soft Matter Physics, Vol. 94, No. 1, 012407, 13.07.2016.

    Research output: Contribution to journalArticle

    Yamamoto, H, Kubota, S, Chida, Y, Morita, M, Moriya, S, Akima, H, Sato, S, Hirano-Iwata, A, Tanii, T & Niwano, M 2016, 'Size-dependent regulation of synchronized activity in living neuronal networks', Physical Review E - Statistical, Nonlinear, and Soft Matter Physics, vol. 94, no. 1, 012407. https://doi.org/10.1103/PhysRevE.94.012407
    Yamamoto, Hideaki ; Kubota, Shigeru ; Chida, Yudai ; Morita, Mayu ; Moriya, Satoshi ; Akima, Hisanao ; Sato, Shigeo ; Hirano-Iwata, Ayumi ; Tanii, Takashi ; Niwano, Michio. / Size-dependent regulation of synchronized activity in living neuronal networks. In: Physical Review E - Statistical, Nonlinear, and Soft Matter Physics. 2016 ; Vol. 94, No. 1.
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