Size-selective separations of biological macromolecules on mesocylinder silica arrays

Sherif El-Safty, M. A. Shenashen

    Research output: Contribution to journalArticle

    39 Citations (Scopus)

    Abstract

    In order to control the design functionality of mesocylinder filters for molecular sieving of proteins, we fabricated tight mesocylinder silica nanotube (NT) arrays as promising filter candidates for size-exclusion separation of high-concentration macromolecules, such as insulin (INS), α-amylase (AMY), β-lactoglobulin (β-LG), and myosin (MYO) proteins. In this study, hexagonal mesocylinder structures were fabricated successfully inside anodic alumina membrane (AAM) nanochannels using a variety of cationic and nonionic surfactants as templates. The systematic design of the nanofilters was based on densely patterned polar silane coupling agents (" linkers" ) onto the AAM nanochannels, leading to the fabrication of mesocylinder silica arrays with vertical alignment and open surfaces of top-bottom ends inside AAM. Further surface coating of silica NTs hybrid AAM with hydrophobic agents facilitated the production of extremely robust constructed sequences of membranes without the formation of air gaps among NT arrays. The fabricated membranes with impermeable coated layers, robust surfaces, and uniformly multidirectional cylinder pores in nanoscale sizes rapidly separate large quantities of proteins within seconds. Meanwhile, comprehensive factors that affect the performance of the molecular transport, diffusivity, and filtration rate through nanofilter membranes were discussed. The mesocylinder filters of macromolecules show promise for the efficient separation and molecular transport of large molecular weight and size as well as concentrations of proteins.

    Original languageEnglish
    Pages (from-to)151-161
    Number of pages11
    JournalAnalytica Chimica Acta
    Volume694
    Issue number1-2
    DOIs
    Publication statusPublished - 2011 May 23

    Fingerprint

    Macromolecules
    Silicon Dioxide
    silica
    membrane
    Membranes
    Aluminum Oxide
    aluminum oxide
    Nanotubes
    protein
    filter
    Proteins
    Silanes
    Lactoglobulins
    sieving
    Cationic surfactants
    Coupling agents
    Nonionic surfactants
    Myosins
    Amylases
    Surface-Active Agents

    Keywords

    • Arrays
    • Biological macromolecules
    • Filter membranes
    • Mesocylinder
    • Permeation flux
    • Size-selective separation

    ASJC Scopus subject areas

    • Biochemistry
    • Analytical Chemistry
    • Spectroscopy
    • Environmental Chemistry

    Cite this

    Size-selective separations of biological macromolecules on mesocylinder silica arrays. / El-Safty, Sherif; Shenashen, M. A.

    In: Analytica Chimica Acta, Vol. 694, No. 1-2, 23.05.2011, p. 151-161.

    Research output: Contribution to journalArticle

    El-Safty, Sherif ; Shenashen, M. A. / Size-selective separations of biological macromolecules on mesocylinder silica arrays. In: Analytica Chimica Acta. 2011 ; Vol. 694, No. 1-2. pp. 151-161.
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