Skewed X-inactivation in cloned mice

Sho Senda, Teruhiko Wakayama, Yukiko Yamazaki, Jun Ohgane, Naka Hattori, Satoshi Tanaka, Ryuzo Yanagimachi, Kunio Shiota

Research output: Contribution to journalArticlepeer-review

30 Citations (Scopus)

Abstract

In female mammals, dosage compensation for X-linked genes is accomplished by inactivation of one of two X chromosomes. The X-inactivation ratio (a percentage of the cells with inactivated maternal X chromosomes in the whole cells) is skewed as a consequence of various genetic mutations, and has been observed in a number of X-linked disorders. We previously reported that phenotypically normal full-term cloned mouse fetuses had loci with inappropriate DNA methylation. Thus, cloned mice are excellent models to study abnormal epigenetic events in mammalian development. In the present study, we analyzed X-inactivation ratios in adult female cloned mice (B6C3F1). Kidneys of eight naturally produced controls and 11 cloned mice were analyzed. Although variations in X-inactivation ratio among the mice were observed in both groups, the distributions were significantly different (Ansary-Bradley test, P

Original languageEnglish
Pages (from-to)38-44
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume321
Issue number1
DOIs
Publication statusPublished - 2004 Aug 13
Externally publishedYes

Keywords

  • Cloned mouse
  • DNA methylation
  • Epigenetics
  • Skewed X-inactivation
  • X-chromosome inactivation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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