Skewed X-inactivation in cloned mice

Sho Senda, Teruhiko Wakayama, Yukiko Yamazaki, Jun Ohgane, Naka Hattori, Satoshi Tanaka, Ryuzo Yanagimachi, Kunio Shiota

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

In female mammals, dosage compensation for X-linked genes is accomplished by inactivation of one of two X chromosomes. The X-inactivation ratio (a percentage of the cells with inactivated maternal X chromosomes in the whole cells) is skewed as a consequence of various genetic mutations, and has been observed in a number of X-linked disorders. We previously reported that phenotypically normal full-term cloned mouse fetuses had loci with inappropriate DNA methylation. Thus, cloned mice are excellent models to study abnormal epigenetic events in mammalian development. In the present study, we analyzed X-inactivation ratios in adult female cloned mice (B6C3F1). Kidneys of eight naturally produced controls and 11 cloned mice were analyzed. Although variations in X-inactivation ratio among the mice were observed in both groups, the distributions were significantly different (Ansary-Bradley test, P

Original languageEnglish
Pages (from-to)38-44
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume321
Issue number1
DOIs
Publication statusPublished - 2004 Aug 13
Externally publishedYes

Fingerprint

X Chromosome Inactivation
Chromosomes
Mammals
X Chromosome
Genes
X-Linked Genes
DNA Methylation
Epigenomics
Fetus
Mothers
Kidney
Mutation
Compensation and Redress

Keywords

  • Cloned mouse
  • DNA methylation
  • Epigenetics
  • Skewed X-inactivation
  • X-chromosome inactivation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Senda, S., Wakayama, T., Yamazaki, Y., Ohgane, J., Hattori, N., Tanaka, S., ... Shiota, K. (2004). Skewed X-inactivation in cloned mice. Biochemical and Biophysical Research Communications, 321(1), 38-44. https://doi.org/10.1016/j.bbrc.2004.06.096

Skewed X-inactivation in cloned mice. / Senda, Sho; Wakayama, Teruhiko; Yamazaki, Yukiko; Ohgane, Jun; Hattori, Naka; Tanaka, Satoshi; Yanagimachi, Ryuzo; Shiota, Kunio.

In: Biochemical and Biophysical Research Communications, Vol. 321, No. 1, 13.08.2004, p. 38-44.

Research output: Contribution to journalArticle

Senda, S, Wakayama, T, Yamazaki, Y, Ohgane, J, Hattori, N, Tanaka, S, Yanagimachi, R & Shiota, K 2004, 'Skewed X-inactivation in cloned mice', Biochemical and Biophysical Research Communications, vol. 321, no. 1, pp. 38-44. https://doi.org/10.1016/j.bbrc.2004.06.096
Senda S, Wakayama T, Yamazaki Y, Ohgane J, Hattori N, Tanaka S et al. Skewed X-inactivation in cloned mice. Biochemical and Biophysical Research Communications. 2004 Aug 13;321(1):38-44. https://doi.org/10.1016/j.bbrc.2004.06.096
Senda, Sho ; Wakayama, Teruhiko ; Yamazaki, Yukiko ; Ohgane, Jun ; Hattori, Naka ; Tanaka, Satoshi ; Yanagimachi, Ryuzo ; Shiota, Kunio. / Skewed X-inactivation in cloned mice. In: Biochemical and Biophysical Research Communications. 2004 ; Vol. 321, No. 1. pp. 38-44.
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