Abstract
In female mammals, dosage compensation for X-linked genes is accomplished by inactivation of one of two X chromosomes. The X-inactivation ratio (a percentage of the cells with inactivated maternal X chromosomes in the whole cells) is skewed as a consequence of various genetic mutations, and has been observed in a number of X-linked disorders. We previously reported that phenotypically normal full-term cloned mouse fetuses had loci with inappropriate DNA methylation. Thus, cloned mice are excellent models to study abnormal epigenetic events in mammalian development. In the present study, we analyzed X-inactivation ratios in adult female cloned mice (B6C3F1). Kidneys of eight naturally produced controls and 11 cloned mice were analyzed. Although variations in X-inactivation ratio among the mice were observed in both groups, the distributions were significantly different (Ansary-Bradley test, P
Original language | English |
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Pages (from-to) | 38-44 |
Number of pages | 7 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 321 |
Issue number | 1 |
DOIs | |
Publication status | Published - 2004 Aug 13 |
Externally published | Yes |
Keywords
- Cloned mouse
- DNA methylation
- Epigenetics
- Skewed X-inactivation
- X-chromosome inactivation
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology