SNP detection in transforming growth factor-β1 gene using bacterial magnetic particles

Hiroyuki Ota, Haruko Takeyama, Hideki Nakayama, Takahiko Katoh, Tadashi Matsunaga

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

A single nucleotide polymorphism (SNP) within the transforming growth factor-β1 (TGF-β1) gene was detected by hybridization-based method using bacterial magnetic particles (BMPs). TGF-β1 is commonly associated with a single base change resulting in a Leu10→Pro (T869→C) polymorphism and is a genetic marker for susceptibility to osteoporosis. Short (9 bases) and specific probes were designed to detect SNP in TGF-β1. Detection probes were immobilized on BMPs using cross-linking reagents. TGF-β1 PCR products (139 bp) were labeled with the fluorescent dye coumarin and hybridized with detection probes on BMPs. Complementary hybridized targets gave over four times higher fluorescent intensities, compared with one base mismatched hybridizations. The SNP genotype was successfully discriminated using this technique.

Original languageEnglish
Pages (from-to)683-687
Number of pages5
JournalBiosensors and Bioelectronics
Volume18
Issue number5-6
DOIs
Publication statusPublished - 2003 May 1
Externally publishedYes

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Keywords

  • Bacterial magnetic particles
  • Hybridization
  • Single nucleotide polymorphism
  • TGF-β1

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Electrochemistry

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