Soft particle analysis of bacterial cells and its interpretation of cell adhesion behaviors in terms of DLVO theory

Hiroshi Hayashi, Satoshi Tsuneda, Akira Hirata, Hiroshi Sasaki

    Research output: Contribution to journalArticle

    92 Citations (Scopus)

    Abstract

    The electrokinetic properties of two nitrifying strains, Nitrosomonas europaea and Nitrobacter winogradskyi, and three heterotrophic bacteria, Escherichia coli, Pseudomonas putida and Pseudomonas aeruginosa, were examined by electrophoretic mobility measurement and analyzed using the soft particle electrophoresis theory that is suitable for biological particles. The bacterial adhesion characteristics onto glass bead substratum were also evaluated by packed bed method. The mobility of the bacterial cells employed converged to a non-zero value as the ionic concentration increased, suggesting that the bacterial cells exhibited typical soft particle characteristics. Moreover, cell surface potentials based on the soft particle theory were lower than those estimated by the conventional Smoluchowski formula, i.e. zeta potential. Cell collision efficiencies onto glass beads (α0) were largely dependent on interfacial interaction, although almost electrically neutral P. aeruginosa did not follow that trend. From a comparison of α0 with DLVO interaction energy maximum (Vmax), it was assumed that heterocoagulation between cell and substratum at primary minimum potential took place under Vmax of 24-34 kT based on soft particle analysis. On the other hand, Vmax predictions using the Smoluchowski theory gave 81-223 kT, which indicated the possibility of overestimating electrostatic repulsive forces by the conventional Smoluchowski theory. Thus, the application of this new electrophoresis theory to several kinds of bacterial cells has led to the revision of the interpretation of bacterial mobility data and provided a more detailed understanding of the bacterial adhesion phenomenon.

    Original languageEnglish
    Pages (from-to)149-157
    Number of pages9
    JournalColloids and Surfaces B: Biointerfaces
    Volume22
    Issue number2
    DOIs
    Publication statusPublished - 2001

    Fingerprint

    Cell adhesion
    Electrophoresis
    Cell Adhesion
    adhesion
    Adhesion
    Glass
    Electrophoretic mobility
    Electrostatic force
    Packed beds
    Surface potential
    Zeta potential
    cells
    Escherichia coli
    Bacterial Adhesion
    Bacteria
    pseudomonas
    nitrobacter
    electrophoresis
    beads
    Pseudomonas aeruginosa

    Keywords

    • Bacterial adhesion
    • DLVO theory
    • Electrophoretic mobility
    • Soft particle electrophoresis theory
    • Surface characteristics

    ASJC Scopus subject areas

    • Biotechnology
    • Colloid and Surface Chemistry
    • Physical and Theoretical Chemistry
    • Surfaces and Interfaces

    Cite this

    Soft particle analysis of bacterial cells and its interpretation of cell adhesion behaviors in terms of DLVO theory. / Hayashi, Hiroshi; Tsuneda, Satoshi; Hirata, Akira; Sasaki, Hiroshi.

    In: Colloids and Surfaces B: Biointerfaces, Vol. 22, No. 2, 2001, p. 149-157.

    Research output: Contribution to journalArticle

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