Soft-X-ray damage to biological samples

H. Fujisaki, S. Takahashi, H. Ohzeki, K. Sugisaki, H. Kondo, H. Nagata, H. Kato, S. Ishiwata

    Research output: Contribution to journalArticle

    9 Citations (Scopus)

    Abstract

    X-ray damage to biological samples was investigated in the wavelength region of 2.7-5 nm, which overlaps the so-called 'water window', the wavelength range of 2.4- 4.3 nm usually used in X-ray microscopy. Yeast cells and myofibrils were chosen as representatives of whole cell samples and motile protein systems, respectively. The samples were exposed to X-rays using an apparatus composed mainly of a laser-plasma X-ray source, a Welter mirror condenser, and a sample cell. The yeast cells lost their dye exclusion ability when the X-ray flux was higher than 1 x 106 photons μm-2, while the myofibrils lost contractility when the X-ray flux was higher than 4 x 105 photons μm-2. These X-ray fluxes are lower than the nux required for the X-ray microscope observation of biological samples at. a resolution higher than that of light microscopes.

    Original languageEnglish
    Pages (from-to)79-83
    Number of pages5
    JournalJournal of Microscopy
    Volume182
    Issue number2
    Publication statusPublished - 1996

    Fingerprint

    X-Rays
    damage
    X rays
    x rays
    Fluxes
    Myofibrils
    yeast
    Yeast
    Photons
    X ray microscopes
    Cells
    Yeasts
    microscopes
    Wavelength
    condensers
    photons
    cells
    exclusion
    laser plasmas
    wavelengths

    Keywords

    • Myofibril
    • Soft X-ray
    • Soft-X-ray damage
    • Water window
    • X-ray
    • X-ray damage
    • X-ray microscope
    • Yeast

    ASJC Scopus subject areas

    • Instrumentation

    Cite this

    Fujisaki, H., Takahashi, S., Ohzeki, H., Sugisaki, K., Kondo, H., Nagata, H., ... Ishiwata, S. (1996). Soft-X-ray damage to biological samples. Journal of Microscopy, 182(2), 79-83.

    Soft-X-ray damage to biological samples. / Fujisaki, H.; Takahashi, S.; Ohzeki, H.; Sugisaki, K.; Kondo, H.; Nagata, H.; Kato, H.; Ishiwata, S.

    In: Journal of Microscopy, Vol. 182, No. 2, 1996, p. 79-83.

    Research output: Contribution to journalArticle

    Fujisaki, H, Takahashi, S, Ohzeki, H, Sugisaki, K, Kondo, H, Nagata, H, Kato, H & Ishiwata, S 1996, 'Soft-X-ray damage to biological samples', Journal of Microscopy, vol. 182, no. 2, pp. 79-83.
    Fujisaki H, Takahashi S, Ohzeki H, Sugisaki K, Kondo H, Nagata H et al. Soft-X-ray damage to biological samples. Journal of Microscopy. 1996;182(2):79-83.
    Fujisaki, H. ; Takahashi, S. ; Ohzeki, H. ; Sugisaki, K. ; Kondo, H. ; Nagata, H. ; Kato, H. ; Ishiwata, S. / Soft-X-ray damage to biological samples. In: Journal of Microscopy. 1996 ; Vol. 182, No. 2. pp. 79-83.
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    AU - Nagata, H.

    AU - Kato, H.

    AU - Ishiwata, S.

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