Soil microbial response to experimental warming in cool temperate semi-natural grassland in Japan

Shimpei Yoshitake, Nozomi Tabei, Yu Mizuno, Hitomi Yoshida, Yuya Sekine, Makoto Tatsumura, Hiroshi Koizumi

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

To assess soil microbial response to global warming in cool temperate semi-natural grassland, we conducted an in situ warming experiment in grassland located in the mountains of central Japan. Five pairs of plots (control and warmed) of Zoysia japonica were established. For each pair of plots, one was warmed by ca. 2 °C using infrared heaters during the growing seasons of 2009–2011. Above-ground biomass of Z.japonica was estimated using the modified point-frame method. Soil organic matter contents, soil total carbon and nitrogen contents, as well as inorganic nitrogen (ammonium and nitrate) contents were determined from soil samples. Total phospholipid fatty acid (PLFA) contents and PLFA compositions were determined and used as indices for total microbial biomass and community structure, respectively. Analyses showed that the warming increased the above-ground biomass of Z. japonica significantly. Soil organic matter and soil total nitrogen contents were significantly decreased, while soil ammonium content was significantly increased in the warmed plots. Soil microbial biomass (especially fungal biomass) was lower in the warmed plots, probably reflecting higher temperature, lower soil water content, and/or depletion in available nutrients. The significant decrease in fungal biomass, in combination with our PLFA composition data, suggests that the soil microbial community structure shifted from a fungal-dominated to a bacteria-dominated one, causing changes in community-level physiological activity.

Original languageEnglish
Pages (from-to)235-245
Number of pages11
JournalEcological Research
Volume30
Issue number2
DOIs
Publication statusPublished - 2015
Externally publishedYes

Fingerprint

warming
grasslands
grassland
Japan
Zoysia japonica
phospholipid
fatty acid composition
soil
fatty acid
aboveground biomass
nitrogen content
biomass
microbial biomass
microbial communities
soil organic matter
community structure
infrared heaters
ammonium
ammonium nitrogen
nitrate nitrogen

Keywords

  • Experimental warming
  • Microbial community structure
  • Pasture
  • Semi-natural grassland
  • Soil microbial biomass

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics

Cite this

Yoshitake, S., Tabei, N., Mizuno, Y., Yoshida, H., Sekine, Y., Tatsumura, M., & Koizumi, H. (2015). Soil microbial response to experimental warming in cool temperate semi-natural grassland in Japan. Ecological Research, 30(2), 235-245. https://doi.org/10.1007/s11284-014-1209-3

Soil microbial response to experimental warming in cool temperate semi-natural grassland in Japan. / Yoshitake, Shimpei; Tabei, Nozomi; Mizuno, Yu; Yoshida, Hitomi; Sekine, Yuya; Tatsumura, Makoto; Koizumi, Hiroshi.

In: Ecological Research, Vol. 30, No. 2, 2015, p. 235-245.

Research output: Contribution to journalArticle

Yoshitake, S, Tabei, N, Mizuno, Y, Yoshida, H, Sekine, Y, Tatsumura, M & Koizumi, H 2015, 'Soil microbial response to experimental warming in cool temperate semi-natural grassland in Japan', Ecological Research, vol. 30, no. 2, pp. 235-245. https://doi.org/10.1007/s11284-014-1209-3
Yoshitake, Shimpei ; Tabei, Nozomi ; Mizuno, Yu ; Yoshida, Hitomi ; Sekine, Yuya ; Tatsumura, Makoto ; Koizumi, Hiroshi. / Soil microbial response to experimental warming in cool temperate semi-natural grassland in Japan. In: Ecological Research. 2015 ; Vol. 30, No. 2. pp. 235-245.
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