Solution structure of the DNA binding domain of a nucleoid-associated protein, H-NS, from Escherichia coli

Heisaburo Shindo, Takanobu Iwaki, Ryoichi Ieda, Hitoshi Kurumizaka, Chiharu Ueguchi, Takeshi Mizuno, Soichi Morikawa, Haruki Nakamura, Hitoshi Kuboniwa

Research output: Contribution to journalArticle

89 Citations (Scopus)

Abstract

The three-dimensional structure of the C-terminal domain (47 residues) obtained from the hydrolysis of H-NS protein with bovine trypsin was determined by NMR measurements and distance geometry calculations. It is composed of an antiparallel β-sheet, an α-helix and a 310-helix which form a hydrophobic core, stabilizing the whole structure. This domain has been found to bind to DNA. Possible DNA binding sites are discussed on the basis of the solution structure of the C-terminal domain of H-NS.

Original languageEnglish
Pages (from-to)125-131
Number of pages7
JournalFEBS Letters
Volume360
Issue number2
DOIs
Publication statusPublished - 1995 Feb 27
Externally publishedYes

Fingerprint

Escherichia coli
DNA
Trypsin
Hydrolysis
Proteins
Binding Sites
Nuclear magnetic resonance
Geometry

Keywords

  • DNA binding protein
  • H-NS
  • NMR
  • Solution structure

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Structural Biology

Cite this

Shindo, H., Iwaki, T., Ieda, R., Kurumizaka, H., Ueguchi, C., Mizuno, T., ... Kuboniwa, H. (1995). Solution structure of the DNA binding domain of a nucleoid-associated protein, H-NS, from Escherichia coli. FEBS Letters, 360(2), 125-131. https://doi.org/10.1016/0014-5793(95)00079-O

Solution structure of the DNA binding domain of a nucleoid-associated protein, H-NS, from Escherichia coli. / Shindo, Heisaburo; Iwaki, Takanobu; Ieda, Ryoichi; Kurumizaka, Hitoshi; Ueguchi, Chiharu; Mizuno, Takeshi; Morikawa, Soichi; Nakamura, Haruki; Kuboniwa, Hitoshi.

In: FEBS Letters, Vol. 360, No. 2, 27.02.1995, p. 125-131.

Research output: Contribution to journalArticle

Shindo, H, Iwaki, T, Ieda, R, Kurumizaka, H, Ueguchi, C, Mizuno, T, Morikawa, S, Nakamura, H & Kuboniwa, H 1995, 'Solution structure of the DNA binding domain of a nucleoid-associated protein, H-NS, from Escherichia coli', FEBS Letters, vol. 360, no. 2, pp. 125-131. https://doi.org/10.1016/0014-5793(95)00079-O
Shindo, Heisaburo ; Iwaki, Takanobu ; Ieda, Ryoichi ; Kurumizaka, Hitoshi ; Ueguchi, Chiharu ; Mizuno, Takeshi ; Morikawa, Soichi ; Nakamura, Haruki ; Kuboniwa, Hitoshi. / Solution structure of the DNA binding domain of a nucleoid-associated protein, H-NS, from Escherichia coli. In: FEBS Letters. 1995 ; Vol. 360, No. 2. pp. 125-131.
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