Solution structure of variant H2A.Z.1 nucleosome investigated by small-angle X-ray and neutron scatterings

Masaaki Sugiyama, Naoki Horikoshi, Yuya Suzuki, Hiroyuki Taguchi, Tomoya Kujirai, Rintaro Inoue, Yojiro Oba, Nobuhiro Sato, Anne Martel, Lionel Porcar, Hitoshi Kurumizaka

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Solution structures of nucleosomes containing a human histone variant, H2A.Z.1, were measured by small-angle X-ray and neutron scatterings (SAXS and SANS). SAXS revealed that the outer shape, reflecting the DNA shape, of the H2A.Z.1 nucleosome is almost the same as that of the canonical H2A nucleosome. In contrast, SANS employing a contrast variation technique revealed that the histone octamer of the H2A.Z.1 nucleosome is smaller than that of the canonical nucleosome. The DNA within the H2A.Z.1 nucleosome was more susceptible to micrococcal nuclease than that within the canonical nucleosome. These results suggested that the DNA is loosely wrapped around the histone core in the H2A.Z.1 nucleosome.

Original languageEnglish
Article number75
Pages (from-to)28-32
Number of pages5
JournalBiochemistry and Biophysics Reports
Volume4
DOIs
Publication statusPublished - 2015 Dec 1

Fingerprint

Nucleosomes
Neutrons
Neutron scattering
X ray scattering
X-Rays
Histones
DNA
Micrococcal Nuclease

Keywords

  • Contrast variation
  • H2A.Z.1
  • Nucleosome
  • Small-angle neutron scattering
  • Small-angle X-ray scattering
  • Stuhrmann plot

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Sugiyama, M., Horikoshi, N., Suzuki, Y., Taguchi, H., Kujirai, T., Inoue, R., ... Kurumizaka, H. (2015). Solution structure of variant H2A.Z.1 nucleosome investigated by small-angle X-ray and neutron scatterings. Biochemistry and Biophysics Reports, 4, 28-32. [75]. https://doi.org/10.1016/j.bbrep.2015.08.019

Solution structure of variant H2A.Z.1 nucleosome investigated by small-angle X-ray and neutron scatterings. / Sugiyama, Masaaki; Horikoshi, Naoki; Suzuki, Yuya; Taguchi, Hiroyuki; Kujirai, Tomoya; Inoue, Rintaro; Oba, Yojiro; Sato, Nobuhiro; Martel, Anne; Porcar, Lionel; Kurumizaka, Hitoshi.

In: Biochemistry and Biophysics Reports, Vol. 4, 75, 01.12.2015, p. 28-32.

Research output: Contribution to journalArticle

Sugiyama, M, Horikoshi, N, Suzuki, Y, Taguchi, H, Kujirai, T, Inoue, R, Oba, Y, Sato, N, Martel, A, Porcar, L & Kurumizaka, H 2015, 'Solution structure of variant H2A.Z.1 nucleosome investigated by small-angle X-ray and neutron scatterings', Biochemistry and Biophysics Reports, vol. 4, 75, pp. 28-32. https://doi.org/10.1016/j.bbrep.2015.08.019
Sugiyama, Masaaki ; Horikoshi, Naoki ; Suzuki, Yuya ; Taguchi, Hiroyuki ; Kujirai, Tomoya ; Inoue, Rintaro ; Oba, Yojiro ; Sato, Nobuhiro ; Martel, Anne ; Porcar, Lionel ; Kurumizaka, Hitoshi. / Solution structure of variant H2A.Z.1 nucleosome investigated by small-angle X-ray and neutron scatterings. In: Biochemistry and Biophysics Reports. 2015 ; Vol. 4. pp. 28-32.
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