Some properties of bovine mitochondrial serine tRNA gene transcript synthesized with T7 RNA polymerase.

Takuya Ueda, K. Watanabe

Research output: Contribution to journalArticle

Abstract

Bovine mitochondrial serine tRNA(AGY) gene transcript was synthesized in vitro with T7 RNA polymerase, and it was capable of being aminoacylated with mitochondrial serine tRNA synthetase. The melting profiles of the transcript was similar to those of native serine tRNA(AGY), suggesting that the higher-order structure of the transcript does not much differ from that of native serine tRNA(AGY). Several transcripts with base-substitution were also constructed and their aminoacylation capacity was investigated.

Original languageEnglish
Pages (from-to)113-114
Number of pages2
JournalNucleic acids symposium series
Issue number21
Publication statusPublished - 1989 Jan 1
Externally publishedYes

Fingerprint

Transfer RNA
Serine
Genes
Aminoacylation
Amino Acyl-tRNA Synthetases
Freezing
bacteriophage T7 RNA polymerase

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Some properties of bovine mitochondrial serine tRNA gene transcript synthesized with T7 RNA polymerase. / Ueda, Takuya; Watanabe, K.

In: Nucleic acids symposium series, No. 21, 01.01.1989, p. 113-114.

Research output: Contribution to journalArticle

@article{dbc948bbc3b442ba8615b4e9ba2fc61c,
title = "Some properties of bovine mitochondrial serine tRNA gene transcript synthesized with T7 RNA polymerase.",
abstract = "Bovine mitochondrial serine tRNA(AGY) gene transcript was synthesized in vitro with T7 RNA polymerase, and it was capable of being aminoacylated with mitochondrial serine tRNA synthetase. The melting profiles of the transcript was similar to those of native serine tRNA(AGY), suggesting that the higher-order structure of the transcript does not much differ from that of native serine tRNA(AGY). Several transcripts with base-substitution were also constructed and their aminoacylation capacity was investigated.",
author = "Takuya Ueda and K. Watanabe",
year = "1989",
month = "1",
day = "1",
language = "English",
pages = "113--114",
journal = "Nucleic acids symposium series",
issn = "0261-3166",
publisher = "Information Retrieval",
number = "21",

}

TY - JOUR

T1 - Some properties of bovine mitochondrial serine tRNA gene transcript synthesized with T7 RNA polymerase.

AU - Ueda, Takuya

AU - Watanabe, K.

PY - 1989/1/1

Y1 - 1989/1/1

N2 - Bovine mitochondrial serine tRNA(AGY) gene transcript was synthesized in vitro with T7 RNA polymerase, and it was capable of being aminoacylated with mitochondrial serine tRNA synthetase. The melting profiles of the transcript was similar to those of native serine tRNA(AGY), suggesting that the higher-order structure of the transcript does not much differ from that of native serine tRNA(AGY). Several transcripts with base-substitution were also constructed and their aminoacylation capacity was investigated.

AB - Bovine mitochondrial serine tRNA(AGY) gene transcript was synthesized in vitro with T7 RNA polymerase, and it was capable of being aminoacylated with mitochondrial serine tRNA synthetase. The melting profiles of the transcript was similar to those of native serine tRNA(AGY), suggesting that the higher-order structure of the transcript does not much differ from that of native serine tRNA(AGY). Several transcripts with base-substitution were also constructed and their aminoacylation capacity was investigated.

UR - http://www.scopus.com/inward/record.url?scp=0024795143&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024795143&partnerID=8YFLogxK

M3 - Article

C2 - 2608455

AN - SCOPUS:0024795143

SP - 113

EP - 114

JO - Nucleic acids symposium series

JF - Nucleic acids symposium series

SN - 0261-3166

IS - 21

ER -