Space shuttling in the cell, Nucleocytoplasmic transport and microtubule organization during the cell cycle

Masamitsu Sato, Takashi Toda

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Microtubules form a multifunctional filamentous structure essential for the cell. In interphase, microtubules form networks in the cytoplasm and play pivotal roles in cell polarity and intracellular transport of various biomolecules. In mitosis, microtubules dramatically change their morphology to assemble the mitotic spindle, thereby pulling the chromosomes toward the spindle poles. One long-standing question is how microtubules are reorganized upon mitotic entry. Yeast cells undergo closed mitosis, in which the nuclear envelope persists, whereas higher eukaryotes undergo open mitosis, in which the nuclear envelope breaks down. Microtubule reorganization must be controlled by selective localization of microtubule-assembly factors. Recent findings in fission yeast indicate that several microtubule-associated proteins (MAPs) shuttle between the cytoplasm and the nucleus through regulation by Ran GTPase, the universal organizer of nucleocytoplasmic transport. Furthermore, the synergistic interplay of Ran and cyclin-dependent kinase (CDK) induces the critical spatiotemporal shift of modes in microtubule assembly from cytoplasmic arrays to nuclear spindles. A MAP complex Alp7/TACCAlp14/TOG undergoes nucleocytoplasmic shuttling in interphase, whereas it is retained in the mitotic nucleus through a decrease of its nuclear export by CDK. Our understanding of how microtubules are reorganized during the cell cycle is beginning to emerge.

Original languageEnglish
Pages (from-to)231-236
Number of pages6
JournalNucleus
Volume1
Issue number3
DOIs
Publication statusPublished - 2010 Jan 1
Externally publishedYes

Keywords

  • Cell cycle
  • Cyclin-dependent kinase (CDK)
  • Microtubule
  • Nuclear transport
  • Ran
  • TACC-TOG

ASJC Scopus subject areas

  • Cell Biology

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