Spatial segregation of polarity factors into distinct cortical clusters is required for cell polarity control

James Dodgson, Anatole Chessel, Miki Yamamoto, Federico Vaggi, Susan Cox, Edward Rosten, David Albrecht, Marco Geymonat, Attila Csikasz-Nagy, Masamitsu Sato, Rafael E. Carazo-Salas

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Cell polarity is regulated by evolutionarily conserved polarity factors whose precise higher-order organization at the cell cortex is largely unknown. Here we image frontally the cortex of live fission yeast cells using time-lapse and super-resolution microscopy. Interestingly, we find that polarity factors are organized in discrete cortical clusters resolvable to ∼50-100 nm in size, which can form and become cortically enriched by oligomerization. We show that forced co-localization of the polarity factors Tea1 and Tea3 results in polarity defects, suggesting that the maintenance of both factors in distinct clusters is required for polarity. However, during mitosis, their co-localization increases, and Tea3 helps to retain the cortical localization of the Tea1 growth landmark in preparation for growth reactivation following mitosis. Thus, regulated spatial segregation of polarity factor clusters provides a means to spatio-temporally control cell polarity at the cell cortex. We observe similar clusters in Saccharomyces cerevisiae and Caenorhabditis elegans cells, indicating this could be a universal regulatory feature.

Original languageEnglish
Article number1834
JournalNature Communications
Volume4
DOIs
Publication statusPublished - 2013
Externally publishedYes

Fingerprint

Cell Polarity
Yeast
polarity
Oligomerization
cells
Mitosis
cortexes
Microscopic examination
Cells
mitosis
Defects
Schizosaccharomyces
Caenorhabditis elegans
Growth
Saccharomyces cerevisiae
Microscopy
Maintenance
saccharomyces
landmarks
yeast

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry(all)
  • Physics and Astronomy(all)

Cite this

Dodgson, J., Chessel, A., Yamamoto, M., Vaggi, F., Cox, S., Rosten, E., ... Carazo-Salas, R. E. (2013). Spatial segregation of polarity factors into distinct cortical clusters is required for cell polarity control. Nature Communications, 4, [1834]. https://doi.org/10.1038/ncomms2813

Spatial segregation of polarity factors into distinct cortical clusters is required for cell polarity control. / Dodgson, James; Chessel, Anatole; Yamamoto, Miki; Vaggi, Federico; Cox, Susan; Rosten, Edward; Albrecht, David; Geymonat, Marco; Csikasz-Nagy, Attila; Sato, Masamitsu; Carazo-Salas, Rafael E.

In: Nature Communications, Vol. 4, 1834, 2013.

Research output: Contribution to journalArticle

Dodgson, J, Chessel, A, Yamamoto, M, Vaggi, F, Cox, S, Rosten, E, Albrecht, D, Geymonat, M, Csikasz-Nagy, A, Sato, M & Carazo-Salas, RE 2013, 'Spatial segregation of polarity factors into distinct cortical clusters is required for cell polarity control', Nature Communications, vol. 4, 1834. https://doi.org/10.1038/ncomms2813
Dodgson, James ; Chessel, Anatole ; Yamamoto, Miki ; Vaggi, Federico ; Cox, Susan ; Rosten, Edward ; Albrecht, David ; Geymonat, Marco ; Csikasz-Nagy, Attila ; Sato, Masamitsu ; Carazo-Salas, Rafael E. / Spatial segregation of polarity factors into distinct cortical clusters is required for cell polarity control. In: Nature Communications. 2013 ; Vol. 4.
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