Spatially focused reagent injection system for cell analysis using 3-D sheath flow scanner

Yasuo Suzuki, Masaki Kanai, Kentaro Kawai, Takahiro Nishimoto, Shuichi Shoji

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

We developed a spatially focused reagent injection system using 3-D sheath flow scanner. The proposed 3-D sheath flow scanner enables to introduce reagent specifically to target cell colonies cultivated in a microchamber. SiO 2 mesh strainer is formed in the microwell to improve spatial resolution of the reagent flow. This enables selective delivery of the reagent to target cell colonies cultivated in the microwell. The specific reagent flow is shifted by eight buffer flows which are controlled by four electro-osmotic flow (EOF) pumps. High performance positioning is realized by PC control with a joystick. Observation of confocal fluorescent image confirmed that the reagent flow is focused to small spot of about 100μmf on the mesh strainer where biological cells are cultivated.

Original languageEnglish
Title of host publicationTRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems
Pages25-28
Number of pages4
DOIs
Publication statusPublished - 2007 Dec 1
Event4th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS '07 - Lyon, France
Duration: 2007 Jun 102007 Jun 14

Publication series

NameTRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems

Conference

Conference4th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS '07
CountryFrance
CityLyon
Period07/6/1007/6/14

    Fingerprint

Keywords

  • 3-D sheath flow
  • Cell analysis
  • EOF pump
  • Micro mesh
  • Sample injector

ASJC Scopus subject areas

  • Control and Systems Engineering
  • Electrical and Electronic Engineering

Cite this

Suzuki, Y., Kanai, M., Kawai, K., Nishimoto, T., & Shoji, S. (2007). Spatially focused reagent injection system for cell analysis using 3-D sheath flow scanner. In TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems (pp. 25-28). [4300063] (TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems). https://doi.org/10.1109/SENSOR.2007.4300063