Spatially focused reagent injection system for cell analysis using 3-D sheath flow scanner

Yasuo Suzuki, Masaki Kanai, Kentaro Kawai, Takahiro Nishimoto, Shuichi Shoji

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    1 Citation (Scopus)

    Abstract

    We developed a spatially focused reagent injection system using 3-D sheath flow scanner. The proposed 3-D sheath flow scanner enables to introduce reagent specifically to target cell colonies cultivated in a microchamber. SiO 2 mesh strainer is formed in the microwell to improve spatial resolution of the reagent flow. This enables selective delivery of the reagent to target cell colonies cultivated in the microwell. The specific reagent flow is shifted by eight buffer flows which are controlled by four electro-osmotic flow (EOF) pumps. High performance positioning is realized by PC control with a joystick. Observation of confocal fluorescent image confirmed that the reagent flow is focused to small spot of about 100μmf on the mesh strainer where biological cells are cultivated.

    Original languageEnglish
    Title of host publicationTRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems
    Pages25-28
    Number of pages4
    DOIs
    Publication statusPublished - 2007
    Event4th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS '07 - Lyon
    Duration: 2007 Jun 102007 Jun 14

    Other

    Other4th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS '07
    CityLyon
    Period07/6/1007/6/14

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    Pumps

    Keywords

    • 3-D sheath flow
    • Cell analysis
    • EOF pump
    • Micro mesh
    • Sample injector

    ASJC Scopus subject areas

    • Control and Systems Engineering
    • Electrical and Electronic Engineering

    Cite this

    Suzuki, Y., Kanai, M., Kawai, K., Nishimoto, T., & Shoji, S. (2007). Spatially focused reagent injection system for cell analysis using 3-D sheath flow scanner. In TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems (pp. 25-28). [4300063] https://doi.org/10.1109/SENSOR.2007.4300063

    Spatially focused reagent injection system for cell analysis using 3-D sheath flow scanner. / Suzuki, Yasuo; Kanai, Masaki; Kawai, Kentaro; Nishimoto, Takahiro; Shoji, Shuichi.

    TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems. 2007. p. 25-28 4300063.

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Suzuki, Y, Kanai, M, Kawai, K, Nishimoto, T & Shoji, S 2007, Spatially focused reagent injection system for cell analysis using 3-D sheath flow scanner. in TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems., 4300063, pp. 25-28, 4th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS '07, Lyon, 07/6/10. https://doi.org/10.1109/SENSOR.2007.4300063
    Suzuki Y, Kanai M, Kawai K, Nishimoto T, Shoji S. Spatially focused reagent injection system for cell analysis using 3-D sheath flow scanner. In TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems. 2007. p. 25-28. 4300063 https://doi.org/10.1109/SENSOR.2007.4300063
    Suzuki, Yasuo ; Kanai, Masaki ; Kawai, Kentaro ; Nishimoto, Takahiro ; Shoji, Shuichi. / Spatially focused reagent injection system for cell analysis using 3-D sheath flow scanner. TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems. 2007. pp. 25-28
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