TY - JOUR
T1 - Spatiotemporal control of cell adhesion on a self-assembled monolayer having a photocleavable protecting group
AU - Nakanishi, Jun
AU - Kikuchi, Yukiko
AU - Takarada, Tohru
AU - Nakayama, Hidekazu
AU - Yamaguchi, Kazuo
AU - Maeda, Mizuo
N1 - Funding Information:
This work was supported by a grant for Ecomolecular Science Research provided by RIKEN (to T.T. and M.M.), and the High-Tech Research Center Project from the Ministry of Education, Culture, Sports, Science, and Technology of Japan (to K.Y.). J.N. thanks the Special Postdoctoral Researcher Program of RIKEN.
PY - 2006/9/18
Y1 - 2006/9/18
N2 - Control of cell adhesion is a key technology for cell-based drug screening and for analyses of cellular processes. We developed a method to spatiotemporally control cell adhesion using a photochemical reaction. We prepared a cell-culturing substrate by modifying the surface of a glass coverslip with a self-assembled monolayer of an alkylsiloxane having a photocleavable 2-nitrobenzyl group. Bovine serum albumin (BSA) was adsorbed onto the substrate to make the surface inert to cell adhesion. When exposed to UV light, the alkylsiloxane underwent a photocleavage reaction, leading to the release of BSA from the surface. Fibronectin, a protein promoting cell adhesion, was added to cover the irradiated regions and made them cell-adhesive. Seeding of cells on this substrate resulted in their selective adhesion to the illuminated regions. By controlling the sizes of the illuminated regions, we formed cell-adhesive spots smaller than single cells and located focal adhesions of the cells. Moreover, by subsequently illuminating the region alongside the cells patterned on the substrate in advance, we released their geometrical confinements and induced migration and proliferation. These manipulations were conducted under a conventional fluorescence microscope without any additional instruments. The present method of cell manipulation will be useful for cell biological studies as well as for the formation of cell arrays.
AB - Control of cell adhesion is a key technology for cell-based drug screening and for analyses of cellular processes. We developed a method to spatiotemporally control cell adhesion using a photochemical reaction. We prepared a cell-culturing substrate by modifying the surface of a glass coverslip with a self-assembled monolayer of an alkylsiloxane having a photocleavable 2-nitrobenzyl group. Bovine serum albumin (BSA) was adsorbed onto the substrate to make the surface inert to cell adhesion. When exposed to UV light, the alkylsiloxane underwent a photocleavage reaction, leading to the release of BSA from the surface. Fibronectin, a protein promoting cell adhesion, was added to cover the irradiated regions and made them cell-adhesive. Seeding of cells on this substrate resulted in their selective adhesion to the illuminated regions. By controlling the sizes of the illuminated regions, we formed cell-adhesive spots smaller than single cells and located focal adhesions of the cells. Moreover, by subsequently illuminating the region alongside the cells patterned on the substrate in advance, we released their geometrical confinements and induced migration and proliferation. These manipulations were conducted under a conventional fluorescence microscope without any additional instruments. The present method of cell manipulation will be useful for cell biological studies as well as for the formation of cell arrays.
KW - Cell adhesion
KW - Cell migration
KW - Cell proliferation
KW - Focal adhesion
KW - Self-assembled monolayer
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U2 - 10.1016/j.aca.2006.04.059
DO - 10.1016/j.aca.2006.04.059
M3 - Article
C2 - 17723700
AN - SCOPUS:33748580290
VL - 578
SP - 100
EP - 104
JO - Analytica Chimica Acta
JF - Analytica Chimica Acta
SN - 0003-2670
IS - 1
ER -