Spectroscopic imaging and the characterization of the autofluorescence properties of human bronchus tissues using UV laser diodes

Masakazu Kobayashi, Rina Sawada, Yasuhiro Ueda

    Research output: Contribution to journalArticle

    2 Citations (Scopus)

    Abstract

    Ultraviolet laser diodes (UV-LD) were used for the excitation source of the autofluorescence (AF) measurements and spectroscopic imaging of the AF originated from the human bronchus was obtained. The AF spectra from the normal bronchus tissues were measured and the clear AF spectrum was obtained by using a short wavelength (400 nm) laser diode; the overlap of the AF signal and excitation source could be substantially eliminated. In order to study the origin of the AF intensity deterioration from the bronchus tissue due to the formation of the tumor tissues, the fluorescence spectrum was measured for various AF substances under various conditions. The blue AF signal of the elastin and NADH solutions which could not be easily studied by the conventional excitation light sources as well as the green AF became weak by adding the lactic acid. The AF spectrum was measured for 512 × 512 pixels and the intensity mapping as a function of the emission wavelength was obtained. Two-dimensional information of the AF signal intensity distribution for a certain wavelength component was measured. The feature originated from the region as small as about 100 μm could be recognized. The numerical calculation of the data was performed and the precise feature of the AF was revealed.

    Original languageEnglish
    Pages (from-to)142-147
    Number of pages6
    JournalIEEE Journal on Selected Topics in Quantum Electronics
    Volume9
    Issue number2
    DOIs
    Publication statusPublished - 2003 Mar

    Fingerprint

    bronchi
    ultraviolet lasers
    Semiconductor lasers
    semiconductor lasers
    Tissue
    Imaging techniques
    Wavelength
    Elastin
    Ultraviolet lasers
    wavelengths
    Lactic acid
    elastin
    excitation
    lactic acid
    Deterioration
    Light sources
    Tumors
    Pixels
    Fluorescence
    deterioration

    Keywords

    • Autofluorescence (AF)
    • Spectroscopic imaging
    • Ultraviolet laser diode (UV-LD)

    ASJC Scopus subject areas

    • Electrical and Electronic Engineering
    • Atomic and Molecular Physics, and Optics

    Cite this

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    abstract = "Ultraviolet laser diodes (UV-LD) were used for the excitation source of the autofluorescence (AF) measurements and spectroscopic imaging of the AF originated from the human bronchus was obtained. The AF spectra from the normal bronchus tissues were measured and the clear AF spectrum was obtained by using a short wavelength (400 nm) laser diode; the overlap of the AF signal and excitation source could be substantially eliminated. In order to study the origin of the AF intensity deterioration from the bronchus tissue due to the formation of the tumor tissues, the fluorescence spectrum was measured for various AF substances under various conditions. The blue AF signal of the elastin and NADH solutions which could not be easily studied by the conventional excitation light sources as well as the green AF became weak by adding the lactic acid. The AF spectrum was measured for 512 × 512 pixels and the intensity mapping as a function of the emission wavelength was obtained. Two-dimensional information of the AF signal intensity distribution for a certain wavelength component was measured. The feature originated from the region as small as about 100 μm could be recognized. The numerical calculation of the data was performed and the precise feature of the AF was revealed.",
    keywords = "Autofluorescence (AF), Spectroscopic imaging, Ultraviolet laser diode (UV-LD)",
    author = "Masakazu Kobayashi and Rina Sawada and Yasuhiro Ueda",
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    AU - Sawada, Rina

    AU - Ueda, Yasuhiro

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    Y1 - 2003/3

    N2 - Ultraviolet laser diodes (UV-LD) were used for the excitation source of the autofluorescence (AF) measurements and spectroscopic imaging of the AF originated from the human bronchus was obtained. The AF spectra from the normal bronchus tissues were measured and the clear AF spectrum was obtained by using a short wavelength (400 nm) laser diode; the overlap of the AF signal and excitation source could be substantially eliminated. In order to study the origin of the AF intensity deterioration from the bronchus tissue due to the formation of the tumor tissues, the fluorescence spectrum was measured for various AF substances under various conditions. The blue AF signal of the elastin and NADH solutions which could not be easily studied by the conventional excitation light sources as well as the green AF became weak by adding the lactic acid. The AF spectrum was measured for 512 × 512 pixels and the intensity mapping as a function of the emission wavelength was obtained. Two-dimensional information of the AF signal intensity distribution for a certain wavelength component was measured. The feature originated from the region as small as about 100 μm could be recognized. The numerical calculation of the data was performed and the precise feature of the AF was revealed.

    AB - Ultraviolet laser diodes (UV-LD) were used for the excitation source of the autofluorescence (AF) measurements and spectroscopic imaging of the AF originated from the human bronchus was obtained. The AF spectra from the normal bronchus tissues were measured and the clear AF spectrum was obtained by using a short wavelength (400 nm) laser diode; the overlap of the AF signal and excitation source could be substantially eliminated. In order to study the origin of the AF intensity deterioration from the bronchus tissue due to the formation of the tumor tissues, the fluorescence spectrum was measured for various AF substances under various conditions. The blue AF signal of the elastin and NADH solutions which could not be easily studied by the conventional excitation light sources as well as the green AF became weak by adding the lactic acid. The AF spectrum was measured for 512 × 512 pixels and the intensity mapping as a function of the emission wavelength was obtained. Two-dimensional information of the AF signal intensity distribution for a certain wavelength component was measured. The feature originated from the region as small as about 100 μm could be recognized. The numerical calculation of the data was performed and the precise feature of the AF was revealed.

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