Spectroscopic studies of autofluorescence substances existing in human tissue

Influences of lactic acid and porphyrins

Yasuhiro Ueda, Masakazu Kobayashi

    Research output: Contribution to journalArticle

    7 Citations (Scopus)

    Abstract

    The influence of lactic acid or porphyrins on the optical properties of tissue fluorophores is investigated by autofluorescence (AF) spectroscopy measurement with a GaN-based ultraviolet laser diode along with Fourier-transform IR (FTIR) spectroscopy measurement. As the lactic-acid concentration becomes dense, the AF peak intensity from elastin and desmosine solutions become wholly weak. A similar reduction in the AF intensity is observed for nicotinamide adenine dinucleotide (NADH) solutions. FTIR analysis indicates that the lactic acid causes the conformational change in elastin and the oxidation of NADH, which can be related to changes in the AF properties. The peak intensity of the tissue fluorophores also becomes weak when porphyrins are added, although the conformational change in each tissue fluorophore is not confirmed from FTIR analysis. Judging from the change in the scattering-light intensity of the excitation source, the observed change mainly originates from the absorption of the excitation source by porphyrins.

    Original languageEnglish
    Pages (from-to)3993-3998
    Number of pages6
    JournalApplied Optics
    Volume43
    Issue number20
    DOIs
    Publication statusPublished - 2004 Jul 10

    Fingerprint

    lactic acid
    Fluorophores
    Porphyrins
    Lactic acid
    porphyrins
    elastin
    Elastin
    Fourier transforms
    Tissue
    Ultraviolet lasers
    nicotinamide
    adenines
    ultraviolet lasers
    Light scattering
    spectroscopy
    luminous intensity
    excitation
    Semiconductor lasers
    Infrared spectroscopy
    Optical properties

    ASJC Scopus subject areas

    • Atomic and Molecular Physics, and Optics

    Cite this

    Spectroscopic studies of autofluorescence substances existing in human tissue : Influences of lactic acid and porphyrins. / Ueda, Yasuhiro; Kobayashi, Masakazu.

    In: Applied Optics, Vol. 43, No. 20, 10.07.2004, p. 3993-3998.

    Research output: Contribution to journalArticle

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