Spermatocyte-specific transcription by calmodulin gene II promoter in transgenic mice

H. Ikeshima, K. Shimoda, K. Matsuo, J. Hata, K. Maejima, T. Takano

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

Transgenic mice carrying a fused gene of the 294-base upstream and 68-base leader sequences of a rat calmodulin gene, CaMII, and β-galactosidase gene were made. Only spermatocytes expressed the transgene mRNA in the testes of four independent transgenic lines. The localization of transgene mRNA was consistent with that of the mouse endogenous CaMII analyzed by in situ hybridization with the probe of 3′-noncoding region of mouse CaMII. Thus, this short promoter of CaMII evidently conferred the expression of transgene only on spermatocytes but not on spermatogonia nor on spermatids of the testis. The rat CaMII promoter up to -294 contained no sequences that corresponded to any of the reported sequence features of genes expressed in the testis. Therefore, this short promoter region of CaMII seemed to carry a certain novel machinery for the spermatocyte-specific gene expression.

Original languageEnglish
Pages (from-to)49-53
Number of pages5
JournalMolecular and Cellular Endocrinology
Volume99
Issue number1
DOIs
Publication statusPublished - 1994
Externally publishedYes

Keywords

  • Calmodulin
  • Gene expression
  • In situ hybridization
  • Spermatocyte
  • Testis
  • Transgenic mouse

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

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