Spindle pole body components are reorganized during fission yeast meiosis

Midori Ohta, Masamitsu Sato, Masayuki Yamamoto

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

During meiosis, the centrosome/spindle pole body (SPB) must be regulated in a manner distinct from that of mitosis to achieve a specialized cell division that will produce gametes. In this paper, we demonstrate that several SPB components are localized to SPBs in a meiosis-specific manner in the fission yeast Schizosaccharomyces pombe. SPB components, such as Cut12, Pcp1, and Spo15, which stay on the SPB during the mitotic cell cycle, disassociate from the SPB during meiotic prophase and then return to the SPB immediately before the onset of meiosis I. Interestingly, the polo kinase Plo1, which normally localizes to the SPB during mitosis, is excluded from them in meiotic prophase, when meiosis-specific, horse-tail nuclear movement occurs. We found that exclusion of Plo1 during this period was essential to properly remodel SPBs, because artificial targeting of Plo1 to SPBs resulted in an overduplication of SPBs. We also found that the centrin Cdc31 was required for meiotic SPB remodeling. Thus Plo1 and a centrin play central roles in the meiotic SPB remodeling, which is essential for generating the proper number of meiotic SPBs and, thereby provide unique characteristics to meiotic divisions.

Original languageEnglish
Pages (from-to)1799-1811
Number of pages13
JournalMolecular Biology of the Cell
Volume23
Issue number10
DOIs
Publication statusPublished - 2012 May 15
Externally publishedYes

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Spindle Pole Bodies
Schizosaccharomyces
Meiosis
Prophase
Spindle Apparatus
Sulfamethoxazole Drug Combination Trimethoprim
Mitosis
Centrosome
Germ Cells
Cell Division
Horses
Tail
Cell Cycle
Phosphotransferases

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Spindle pole body components are reorganized during fission yeast meiosis. / Ohta, Midori; Sato, Masamitsu; Yamamoto, Masayuki.

In: Molecular Biology of the Cell, Vol. 23, No. 10, 15.05.2012, p. 1799-1811.

Research output: Contribution to journalArticle

Ohta, Midori ; Sato, Masamitsu ; Yamamoto, Masayuki. / Spindle pole body components are reorganized during fission yeast meiosis. In: Molecular Biology of the Cell. 2012 ; Vol. 23, No. 10. pp. 1799-1811.
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