Spindle pole body movement is affected by glucose and ammonium chloride in fission yeast

Hiroaki Ito, Takeshi Sugawara, Soya Shinkai, Satoshi Mizukawa, Ayaka Kondo, Hisamichi Senda, Kengo Sawai, Koki Ito, Sayaka Suzuki, Masakatsu Takaine, Satoshi Yoshida, Hiromi Imamura, Kenji Kitamura, Toshinori Namba, Shin ichi Tate, Masaru Ueno

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The complexity of chromatin dynamics is orchestrated by several active processes. In fission yeast, the centromeres are clustered around the spindle pole body (SPB) and oscillate in a microtubule- and adenosine triphosphate (ATP)-dependent manner. However, whether and how SPB oscillation are affected by different environmental conditions remain poorly understood. In this study, we quantitated movements of the SPB component, which colocalizes with the centromere in fission yeast. We found that SPB movement was significantly reduced at low glucose concentrations. Movement of the SPB was also affected by the presence of ammonium chloride. Power spectral analysis revealed that periodic movement of the SPB is disrupted by low glucose concentrations. Measurement of ATP levels in living cells by quantitative single-cell imaging suggests that ATP levels are not the only determinant of SPB movement. Our results provide novel insight into how SPB movement is regulated by cellular energy status and additional factors such as the medium nutritional composition.

Original languageEnglish
Pages (from-to)820-825
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume511
Issue number4
DOIs
Publication statusPublished - 2019 Apr 16

Fingerprint

Spindle Pole Bodies
Ammonium Chloride
Schizosaccharomyces
Yeast
Poles
Glucose
Centromere
Adenosine Triphosphate
Microtubules
Spectrum analysis
Chromatin
Cells
Imaging techniques

Keywords

  • Adenosine triphosphate
  • Ammonium chloride
  • Fission yeast
  • Glucose
  • Spindle pole body dynamics

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Spindle pole body movement is affected by glucose and ammonium chloride in fission yeast. / Ito, Hiroaki; Sugawara, Takeshi; Shinkai, Soya; Mizukawa, Satoshi; Kondo, Ayaka; Senda, Hisamichi; Sawai, Kengo; Ito, Koki; Suzuki, Sayaka; Takaine, Masakatsu; Yoshida, Satoshi; Imamura, Hiromi; Kitamura, Kenji; Namba, Toshinori; Tate, Shin ichi; Ueno, Masaru.

In: Biochemical and Biophysical Research Communications, Vol. 511, No. 4, 16.04.2019, p. 820-825.

Research output: Contribution to journalArticle

Ito, H, Sugawara, T, Shinkai, S, Mizukawa, S, Kondo, A, Senda, H, Sawai, K, Ito, K, Suzuki, S, Takaine, M, Yoshida, S, Imamura, H, Kitamura, K, Namba, T, Tate, SI & Ueno, M 2019, 'Spindle pole body movement is affected by glucose and ammonium chloride in fission yeast', Biochemical and Biophysical Research Communications, vol. 511, no. 4, pp. 820-825. https://doi.org/10.1016/j.bbrc.2019.02.128
Ito, Hiroaki ; Sugawara, Takeshi ; Shinkai, Soya ; Mizukawa, Satoshi ; Kondo, Ayaka ; Senda, Hisamichi ; Sawai, Kengo ; Ito, Koki ; Suzuki, Sayaka ; Takaine, Masakatsu ; Yoshida, Satoshi ; Imamura, Hiromi ; Kitamura, Kenji ; Namba, Toshinori ; Tate, Shin ichi ; Ueno, Masaru. / Spindle pole body movement is affected by glucose and ammonium chloride in fission yeast. In: Biochemical and Biophysical Research Communications. 2019 ; Vol. 511, No. 4. pp. 820-825.
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