Splenic macrophages enhance prolactin-induced progestin secretion from mature rat granulosa cells in vitro

K. Yamanouchi, S. Matsuyama, M. Nishihara, K. Shiota, C. Tachi, M. Takahashi

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Abstract

The effect of splenic macrophages on in vitro progestin secretion (the sum of the progesterone and 4-pregnen-20α-o1-3-one concentrations in the medium) from mature rat granulosa cells was examined by means of co-culture techniques. When splenic macrophages (3.0 x 105 cells/ml) obtained from adult female rats on the evening of proestrus (1800 h) were added to granulosa cells (1.5 x 105 cells/ml) and co-cultured for 96 h in the absence of prolactin (PRL), progestin secretion from granulosa cells did not change. However, co-culture of granulosa cells with the macrophages in the presence of PRL (2 μg/ml) significantly enhanced progestin secretion after 48 h of culture. This stimulatory effect on progestin secretion was observed only when the number of macrophages added was more than twice the number of granulosa cells. On the other hand, splenic macrophages obtained on the evening of diestrus had no effect on progestin secretion from granulosa cells even in the presence of PRL. These results suggest that splenic macrophages can enhance PRL action so as to stimulate progestin secretion from granulosa cells and that this function of splenic macrophages varies during the estrous cycle.

Original languageEnglish
Pages (from-to)1109-1113
Number of pages5
JournalBiology of Reproduction
Volume46
Issue number6
DOIs
Publication statusPublished - 1992
Externally publishedYes

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ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Embryology

Cite this

Yamanouchi, K., Matsuyama, S., Nishihara, M., Shiota, K., Tachi, C., & Takahashi, M. (1992). Splenic macrophages enhance prolactin-induced progestin secretion from mature rat granulosa cells in vitro. Biology of Reproduction, 46(6), 1109-1113. https://doi.org/10.1095/biolreprod46.6.1109