Spontaneous oscillation of tension and sarcomere length in skeletal myofibrils. Microscopic measurement and analysis

T. Anazawa, Kenji Yasuda, S. Ishiwata

    Research output: Contribution to journalArticle

    48 Citations (Scopus)

    Abstract

    We have devised a simple method for measuring tension development of single myofibrils by micromanipulation with a pair of glass micro-needles. The tension was estimated from the deflection of a flexible needle under an inverted phase-contrast microscope equipped with an image processor, so that the tension development is always accompanied by the shortening of the myofibril (auxotonic condition) in the present setup. The advantage of this method is that the measurement of tension (1/30 s for time resolution and about 0.05 μg for accuracy of tension measurement; 0.05 μm as a spatial resolution for displacement of the micro-needle) and the observation of sarcomere structure are possible at the same time, and the technique to hold myofibrils, even single myofibrils, is very simple. This method has been applied to study the tension development of glycerinated skeletal myofibrils under the condition where spontaneous oscillation of sarcomeres is induced, i.e., the coexistence of MgATP, MgADP and inorganic phosphate without free Ca2+. Under this condition, we found that the tension of myofibrils spontaneously oscillates accompanied by the oscillation of sarcomere length with a main period of a few seconds; the period was lengthened and shortened with stretch and release of myofibrils. A possible mechanism of the oscillation is discussed.

    Original languageEnglish
    Pages (from-to)1099-1108
    Number of pages10
    JournalBiophysical Journal
    Volume61
    Issue number5
    Publication statusPublished - 1992

    Fingerprint

    Sarcomeres
    Myofibrils
    Needles
    Micromanipulation
    Adenosine Diphosphate
    Glass
    Adenosine Triphosphate
    Phosphates
    Observation

    ASJC Scopus subject areas

    • Biophysics

    Cite this

    Spontaneous oscillation of tension and sarcomere length in skeletal myofibrils. Microscopic measurement and analysis. / Anazawa, T.; Yasuda, Kenji; Ishiwata, S.

    In: Biophysical Journal, Vol. 61, No. 5, 1992, p. 1099-1108.

    Research output: Contribution to journalArticle

    @article{ad5660f013c841389532cfc214debac8,
    title = "Spontaneous oscillation of tension and sarcomere length in skeletal myofibrils. Microscopic measurement and analysis",
    abstract = "We have devised a simple method for measuring tension development of single myofibrils by micromanipulation with a pair of glass micro-needles. The tension was estimated from the deflection of a flexible needle under an inverted phase-contrast microscope equipped with an image processor, so that the tension development is always accompanied by the shortening of the myofibril (auxotonic condition) in the present setup. The advantage of this method is that the measurement of tension (1/30 s for time resolution and about 0.05 μg for accuracy of tension measurement; 0.05 μm as a spatial resolution for displacement of the micro-needle) and the observation of sarcomere structure are possible at the same time, and the technique to hold myofibrils, even single myofibrils, is very simple. This method has been applied to study the tension development of glycerinated skeletal myofibrils under the condition where spontaneous oscillation of sarcomeres is induced, i.e., the coexistence of MgATP, MgADP and inorganic phosphate without free Ca2+. Under this condition, we found that the tension of myofibrils spontaneously oscillates accompanied by the oscillation of sarcomere length with a main period of a few seconds; the period was lengthened and shortened with stretch and release of myofibrils. A possible mechanism of the oscillation is discussed.",
    author = "T. Anazawa and Kenji Yasuda and S. Ishiwata",
    year = "1992",
    language = "English",
    volume = "61",
    pages = "1099--1108",
    journal = "Biophysical Journal",
    issn = "0006-3495",
    publisher = "Biophysical Society",
    number = "5",

    }

    TY - JOUR

    T1 - Spontaneous oscillation of tension and sarcomere length in skeletal myofibrils. Microscopic measurement and analysis

    AU - Anazawa, T.

    AU - Yasuda, Kenji

    AU - Ishiwata, S.

    PY - 1992

    Y1 - 1992

    N2 - We have devised a simple method for measuring tension development of single myofibrils by micromanipulation with a pair of glass micro-needles. The tension was estimated from the deflection of a flexible needle under an inverted phase-contrast microscope equipped with an image processor, so that the tension development is always accompanied by the shortening of the myofibril (auxotonic condition) in the present setup. The advantage of this method is that the measurement of tension (1/30 s for time resolution and about 0.05 μg for accuracy of tension measurement; 0.05 μm as a spatial resolution for displacement of the micro-needle) and the observation of sarcomere structure are possible at the same time, and the technique to hold myofibrils, even single myofibrils, is very simple. This method has been applied to study the tension development of glycerinated skeletal myofibrils under the condition where spontaneous oscillation of sarcomeres is induced, i.e., the coexistence of MgATP, MgADP and inorganic phosphate without free Ca2+. Under this condition, we found that the tension of myofibrils spontaneously oscillates accompanied by the oscillation of sarcomere length with a main period of a few seconds; the period was lengthened and shortened with stretch and release of myofibrils. A possible mechanism of the oscillation is discussed.

    AB - We have devised a simple method for measuring tension development of single myofibrils by micromanipulation with a pair of glass micro-needles. The tension was estimated from the deflection of a flexible needle under an inverted phase-contrast microscope equipped with an image processor, so that the tension development is always accompanied by the shortening of the myofibril (auxotonic condition) in the present setup. The advantage of this method is that the measurement of tension (1/30 s for time resolution and about 0.05 μg for accuracy of tension measurement; 0.05 μm as a spatial resolution for displacement of the micro-needle) and the observation of sarcomere structure are possible at the same time, and the technique to hold myofibrils, even single myofibrils, is very simple. This method has been applied to study the tension development of glycerinated skeletal myofibrils under the condition where spontaneous oscillation of sarcomeres is induced, i.e., the coexistence of MgATP, MgADP and inorganic phosphate without free Ca2+. Under this condition, we found that the tension of myofibrils spontaneously oscillates accompanied by the oscillation of sarcomere length with a main period of a few seconds; the period was lengthened and shortened with stretch and release of myofibrils. A possible mechanism of the oscillation is discussed.

    UR - http://www.scopus.com/inward/record.url?scp=0026752527&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=0026752527&partnerID=8YFLogxK

    M3 - Article

    VL - 61

    SP - 1099

    EP - 1108

    JO - Biophysical Journal

    JF - Biophysical Journal

    SN - 0006-3495

    IS - 5

    ER -