Src-homology domain 2 is responsible for transcriptional suppression induced by expression of Lck

M. Ohta, H. Nagai, H. Nyunoya, K. Shimotohno

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Overexpression of Lck was shown, by our previous study, to suppress gene transcription from various viral and cellular promoters. The suppression of transcription from human T-cell leukemia virus promoter by Lck was independent of the presence of the enhancer core sequences within the long terminal repeat. The suppression of transcription was observed with Lck mutants that had either diminished or enhanced tyrosine-kinase activity. A mutant lacking the myristylation site also suppressed transcription. From the analysis with various deletion mutants of Lck, it was suggested that Src-homology domain 2 (SH2) is both necessary and sufficient for the suppression of transcription. A similar effect was also observed with the SH2 domain of the v-src gene. Thus, overexpression of Lck could suppress gene expression through a unique function of the SH2 domain.

Original languageEnglish
Pages (from-to)989-997
Number of pages9
JournalOncogene
Volume12
Issue number5
Publication statusPublished - 1996 Apr 17
Externally publishedYes

Keywords

  • Lck
  • SH2
  • Src
  • Transcriptional suppression
  • Tyrosine kinase

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

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