Stable complex formation of CENP-B with the CENP-A nucleosome

Risa Fujita, Koichiro Otake, Yasuhiro Arimura, Naoki Horikoshi, Yuta Miya, Tatsuya Shiga, Akihisa Osakabe, Hiroaki Tachiwana, Jun Ichirou Ohzeki, Vladimir Larionov, Hiroshi Masumoto, Hitoshi Kurumizaka

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28 Citations (Scopus)

Abstract

CENP-A and CENP-B are major components of centromeric chromatin. CENP-A is the histone H3 variant, which forms the centromere-specific nucleosome. CENP-B specifically binds to the CENP-B box DNA sequence on the centromere-specific repetitive DNA. In the present study, we found that the CENP-A nucleosome more stably retains human CENP-B than the H3.1 nucleosome in vitro. Specifically, CENP-B forms a stable complex with the CENP-A nucleosome, when the CENP-B box sequence is located at the proximal edge of the nucleosome. Surprisingly, the CENP-B binding was weaker when the CENP-B box sequence was located in the distal linker region of the nucleosome. This difference in CENP-B binding, depending on the CENP-B box location, was not observed with the H3.1 nucleosome. Consistently, we found that the DNA-binding domain of CENP-B specifically interacted with the CENP-A-H4 complex, but not with the H3.1-H4 complex, in vitro. These results suggested that CENP-B forms a more stable complex with the CENP-A nucleosome through specific interactions with CENP-A, if the CENP-B box is located proximal to the CENP-A nucleosome. Our in vivo assay also revealed that CENP-B binding in the vicinity of the CENP-A nucleosome substantially stabilizes the CENP-A nucleosome on alphoid DNA in human cells.

Original languageEnglish
Pages (from-to)4909-4922
Number of pages14
JournalNucleic Acids Research
Volume43
Issue number10
DOIs
Publication statusPublished - 2015 Apr 15

ASJC Scopus subject areas

  • Genetics

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    Fujita, R., Otake, K., Arimura, Y., Horikoshi, N., Miya, Y., Shiga, T., Osakabe, A., Tachiwana, H., Ohzeki, J. I., Larionov, V., Masumoto, H., & Kurumizaka, H. (2015). Stable complex formation of CENP-B with the CENP-A nucleosome. Nucleic Acids Research, 43(10), 4909-4922. https://doi.org/10.1093/nar/gkv405