Stable complex formation of CENP-B with the CENP-A nucleosome

Risa Fujita, Koichiro Otake, Yasuhiro Arimura, Naoki Horikoshi, Yuta Miya, Tatsuya Shiga, Akihisa Osakabe, Hiroaki Tachiwana, Jun Ichirou Ohzeki, Vladimir Larionov, Hiroshi Masumoto, Hitoshi Kurumizaka

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

CENP-A and CENP-B are major components of centromeric chromatin. CENP-A is the histone H3 variant, which forms the centromere-specific nucleosome. CENP-B specifically binds to the CENP-B box DNA sequence on the centromere-specific repetitive DNA. In the present study, we found that the CENP-A nucleosome more stably retains human CENP-B than the H3.1 nucleosome in vitro. Specifically, CENP-B forms a stable complex with the CENP-A nucleosome, when the CENP-B box sequence is located at the proximal edge of the nucleosome. Surprisingly, the CENP-B binding was weaker when the CENP-B box sequence was located in the distal linker region of the nucleosome. This difference in CENP-B binding, depending on the CENP-B box location, was not observed with the H3.1 nucleosome. Consistently, we found that the DNA-binding domain of CENP-B specifically interacted with the CENP-A-H4 complex, but not with the H3.1-H4 complex, in vitro. These results suggested that CENP-B forms a more stable complex with the CENP-A nucleosome through specific interactions with CENP-A, if the CENP-B box is located proximal to the CENP-A nucleosome. Our in vivo assay also revealed that CENP-B binding in the vicinity of the CENP-A nucleosome substantially stabilizes the CENP-A nucleosome on alphoid DNA in human cells.

Original languageEnglish
Pages (from-to)4909-4922
Number of pages14
JournalNucleic Acids Research
Volume43
Issue number10
DOIs
Publication statusPublished - 2015 Apr 15

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Nucleosomes
Centromere
DNA
centromere protein A
Histones
Chromatin

ASJC Scopus subject areas

  • Genetics

Cite this

Fujita, R., Otake, K., Arimura, Y., Horikoshi, N., Miya, Y., Shiga, T., ... Kurumizaka, H. (2015). Stable complex formation of CENP-B with the CENP-A nucleosome. Nucleic Acids Research, 43(10), 4909-4922. https://doi.org/10.1093/nar/gkv405

Stable complex formation of CENP-B with the CENP-A nucleosome. / Fujita, Risa; Otake, Koichiro; Arimura, Yasuhiro; Horikoshi, Naoki; Miya, Yuta; Shiga, Tatsuya; Osakabe, Akihisa; Tachiwana, Hiroaki; Ohzeki, Jun Ichirou; Larionov, Vladimir; Masumoto, Hiroshi; Kurumizaka, Hitoshi.

In: Nucleic Acids Research, Vol. 43, No. 10, 15.04.2015, p. 4909-4922.

Research output: Contribution to journalArticle

Fujita, R, Otake, K, Arimura, Y, Horikoshi, N, Miya, Y, Shiga, T, Osakabe, A, Tachiwana, H, Ohzeki, JI, Larionov, V, Masumoto, H & Kurumizaka, H 2015, 'Stable complex formation of CENP-B with the CENP-A nucleosome', Nucleic Acids Research, vol. 43, no. 10, pp. 4909-4922. https://doi.org/10.1093/nar/gkv405
Fujita R, Otake K, Arimura Y, Horikoshi N, Miya Y, Shiga T et al. Stable complex formation of CENP-B with the CENP-A nucleosome. Nucleic Acids Research. 2015 Apr 15;43(10):4909-4922. https://doi.org/10.1093/nar/gkv405
Fujita, Risa ; Otake, Koichiro ; Arimura, Yasuhiro ; Horikoshi, Naoki ; Miya, Yuta ; Shiga, Tatsuya ; Osakabe, Akihisa ; Tachiwana, Hiroaki ; Ohzeki, Jun Ichirou ; Larionov, Vladimir ; Masumoto, Hiroshi ; Kurumizaka, Hitoshi. / Stable complex formation of CENP-B with the CENP-A nucleosome. In: Nucleic Acids Research. 2015 ; Vol. 43, No. 10. pp. 4909-4922.
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