Stage-specific modification of G protein beta subunits in rat placenta

Ryuta Ishimura, Kenji Yoshida, Hiromichi Kimura, Naoshi Dohmae, Koji Takio, Tomoya Ogawa, Satoshi Tanaka, Kunio Shiota

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We previously analysed the plasma membrane proteins of rat placenta and prepared a database of 150 plasma membrane proteins, expressed in a stage-specific manner, utilizing two-dimensional gel electrophoresis (2D/E) [Mol. Cell. Endocrinol. 115(1995)149]. In this study, we focused on the proteins, tentatively named psL-I (MW 36.2 kDa, pI 5.3) and psL-II (35.9 kDa, 5.3), which were expressed mainly in late pregnancy. Close to psL-I and psL-II on 2D/E gels, we also recognized more abundant proteins [psC-I (36.2 kDa, 5.4) and psC-II (35.9 kDa, 5.4), respectively] arranged side by side with the same MW but different pI. Expression of psL-I and psL-II was detected only in junctional zone of placenta, whereas psC-I and psC-II were expressed in both labyrinth and junctional zones. In addition, psL-I and psL-II began to increase on day 16 of pregnancy and peaked at term, whereas expression of psC-I and psC-II was relatively constant. The analysis of these four proteins (psL-I, psL-II, psC-I and psC-II) by preparative 2D/E, peptide mapping, amino acid sequence and mass spectrometry (MALDI-TOF-MS) revealed that psC-I was a G protein β1 subunit, and psC-II was a β2 subunit, and showed that psL-I and psL-II were molecular modified forms of psC-I and psC-II, respectively. Expression of these G protein β subunits (psL-I, psL-II, psC-I and psC-II) was also observed in rat choriocarcinoma cells, Rcho-1 cells. Expression of psC-I and psC-II was much higher than those of psL-I and psL-II, and their level was relatively constant regardless of the stage of differentiation in vitro. Interestingly, expression of psL-I and psL-II gradually increased in association with the differentiation. Since the expression of β1 and β2 subunit proteins and their mRNAs was constant during the process of differentiation in Rcho-1 cells, the expression of these lower pI forms of G protein subunits (psL-I and psL-II) was thought to be post-translationally regulated. In conclusion, there are modified forms of G protein β1 and β2 subunits, in the placenta and Rcho-1 cells, which are expressed in a pregnancy-stage or differentiation stage specific manner.

Original languageEnglish
Pages (from-to)77-89
Number of pages13
JournalMolecular and Cellular Endocrinology
Volume174
Issue number1-2
DOIs
Publication statusPublished - 2001 Mar 28
Externally publishedYes

Fingerprint

GTP-Binding Protein beta Subunits
GTP-Binding Proteins
Placenta
Protein Subunits
Rats
Cell membranes
Membrane Proteins
Pregnancy
Gels
Blood Proteins
Proteins
Cell Membrane
Electrophoresis
Choriocarcinoma
Peptide Mapping
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Mass spectrometry
Electrophoresis, Gel, Two-Dimensional
Inner Ear
Amino Acid Sequence

Keywords

  • Placenta
  • Post-translational modification
  • Rat
  • Two-dimensional gel electrophoresis

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Ishimura, R., Yoshida, K., Kimura, H., Dohmae, N., Takio, K., Ogawa, T., ... Shiota, K. (2001). Stage-specific modification of G protein beta subunits in rat placenta. Molecular and Cellular Endocrinology, 174(1-2), 77-89. https://doi.org/10.1016/S0303-7207(00)00451-2

Stage-specific modification of G protein beta subunits in rat placenta. / Ishimura, Ryuta; Yoshida, Kenji; Kimura, Hiromichi; Dohmae, Naoshi; Takio, Koji; Ogawa, Tomoya; Tanaka, Satoshi; Shiota, Kunio.

In: Molecular and Cellular Endocrinology, Vol. 174, No. 1-2, 28.03.2001, p. 77-89.

Research output: Contribution to journalArticle

Ishimura, R, Yoshida, K, Kimura, H, Dohmae, N, Takio, K, Ogawa, T, Tanaka, S & Shiota, K 2001, 'Stage-specific modification of G protein beta subunits in rat placenta', Molecular and Cellular Endocrinology, vol. 174, no. 1-2, pp. 77-89. https://doi.org/10.1016/S0303-7207(00)00451-2
Ishimura, Ryuta ; Yoshida, Kenji ; Kimura, Hiromichi ; Dohmae, Naoshi ; Takio, Koji ; Ogawa, Tomoya ; Tanaka, Satoshi ; Shiota, Kunio. / Stage-specific modification of G protein beta subunits in rat placenta. In: Molecular and Cellular Endocrinology. 2001 ; Vol. 174, No. 1-2. pp. 77-89.
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AU - Kimura, Hiromichi

AU - Dohmae, Naoshi

AU - Takio, Koji

AU - Ogawa, Tomoya

AU - Tanaka, Satoshi

AU - Shiota, Kunio

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N2 - We previously analysed the plasma membrane proteins of rat placenta and prepared a database of 150 plasma membrane proteins, expressed in a stage-specific manner, utilizing two-dimensional gel electrophoresis (2D/E) [Mol. Cell. Endocrinol. 115(1995)149]. In this study, we focused on the proteins, tentatively named psL-I (MW 36.2 kDa, pI 5.3) and psL-II (35.9 kDa, 5.3), which were expressed mainly in late pregnancy. Close to psL-I and psL-II on 2D/E gels, we also recognized more abundant proteins [psC-I (36.2 kDa, 5.4) and psC-II (35.9 kDa, 5.4), respectively] arranged side by side with the same MW but different pI. Expression of psL-I and psL-II was detected only in junctional zone of placenta, whereas psC-I and psC-II were expressed in both labyrinth and junctional zones. In addition, psL-I and psL-II began to increase on day 16 of pregnancy and peaked at term, whereas expression of psC-I and psC-II was relatively constant. The analysis of these four proteins (psL-I, psL-II, psC-I and psC-II) by preparative 2D/E, peptide mapping, amino acid sequence and mass spectrometry (MALDI-TOF-MS) revealed that psC-I was a G protein β1 subunit, and psC-II was a β2 subunit, and showed that psL-I and psL-II were molecular modified forms of psC-I and psC-II, respectively. Expression of these G protein β subunits (psL-I, psL-II, psC-I and psC-II) was also observed in rat choriocarcinoma cells, Rcho-1 cells. Expression of psC-I and psC-II was much higher than those of psL-I and psL-II, and their level was relatively constant regardless of the stage of differentiation in vitro. Interestingly, expression of psL-I and psL-II gradually increased in association with the differentiation. Since the expression of β1 and β2 subunit proteins and their mRNAs was constant during the process of differentiation in Rcho-1 cells, the expression of these lower pI forms of G protein subunits (psL-I and psL-II) was thought to be post-translationally regulated. In conclusion, there are modified forms of G protein β1 and β2 subunits, in the placenta and Rcho-1 cells, which are expressed in a pregnancy-stage or differentiation stage specific manner.

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