Stand-alone micro fluidic system using partly disposable PDMS microwell array for high throughput cell analysis

Kasumi Miyamoto; Ryo Yamamoto; Kentaro Kawai; Shuichi Shoji

doi:10.1109/TRANSDUCERS.2011.5969418

Stand-alone micro fluidic system using partly disposable PDMS microwell array for high throughput cell analysis

Kasumi Miyamoto, Ryo Yamamoto^*, Kentaro Kawai, Shuichi Shoji

^*Corresponding author for this work

School of Fundamental Science and Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

A stand-alone microfluidic system consists of a reusable flow control part and a disposable microwell array for high throughput cell analysis is proposed. The flow control part is realized with addressable microvalve array driven by compactly-fabricated solenoid actuators and EOF micropumps. Disposable 16 microwell array with 4 reagent injection ports was fabricated using a PDMS structure. The disposable fluidic chip can be easily replaced to different functional fluidic networks depended on their actual uses. The prototype system with 2×5 (5²) on-chip hydraulic valves that are driven by 10 (25) solenoid actuators was fabricated. Precise reagent injection of ten-nanoliter order to each microwell was successfully carried out.

Original language	English
Title of host publication	2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11
Pages	2944-2947
Number of pages	4
DOIs	https://doi.org/10.1109/TRANSDUCERS.2011.5969418
Publication status	Published - 2011
Event	2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11 - Beijing, China Duration: 2011 Jun 5 → 2011 Jun 9

Publication series

Name	2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11

Conference

Conference	2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11
Country/Territory	China
City	Beijing
Period	11/6/5 → 11/6/9

Keywords

Access-tube-free
Addressable valve control system
Hydraulic valve
Partly disposable
Stand-alone

ASJC Scopus subject areas

Hardware and Architecture
Electrical and Electronic Engineering

Access to Document

10.1109/TRANSDUCERS.2011.5969418

Cite this

Miyamoto, K., Yamamoto, R., Kawai, K., & Shoji, S. (2011). Stand-alone micro fluidic system using partly disposable PDMS microwell array for high throughput cell analysis. In 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11 (pp. 2944-2947). [5969418] (2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11). https://doi.org/10.1109/TRANSDUCERS.2011.5969418

Stand-alone micro fluidic system using partly disposable PDMS microwell array for high throughput cell analysis. / Miyamoto, Kasumi; Yamamoto, Ryo; Kawai, Kentaro et al.

2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11. 2011. p. 2944-2947 5969418 (2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Miyamoto, K, Yamamoto, R, Kawai, K & Shoji, S 2011, Stand-alone micro fluidic system using partly disposable PDMS microwell array for high throughput cell analysis. in 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11., 5969418, 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11, pp. 2944-2947, 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11, Beijing, China, 11/6/5. https://doi.org/10.1109/TRANSDUCERS.2011.5969418

Miyamoto K, Yamamoto R, Kawai K, Shoji S. Stand-alone micro fluidic system using partly disposable PDMS microwell array for high throughput cell analysis. In 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11. 2011. p. 2944-2947. 5969418. (2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11). doi: 10.1109/TRANSDUCERS.2011.5969418

Miyamoto, Kasumi ; Yamamoto, Ryo ; Kawai, Kentaro et al. / Stand-alone micro fluidic system using partly disposable PDMS microwell array for high throughput cell analysis. 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11. 2011. pp. 2944-2947 (2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11).

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keywords = "Access-tube-free, Addressable valve control system, Hydraulic valve, Partly disposable, Stand-alone",

author = "Kasumi Miyamoto and Ryo Yamamoto and Kentaro Kawai and Shuichi Shoji",

note = "Funding Information: Fabrication process has been partly carried out at Waseda University Nanotechnology Research Center (NTRC). This work is partly supported by Japan Ministry of Education, Culture, Sports Science and Technology Grant-in-Aid for Scientific Basic Research (S) No. 23226010 of Waseda University, Global COE program “Ambient GCOE” of Waseda University. ; 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11 ; Conference date: 05-06-2011 Through 09-06-2011",

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N1 - Funding Information: Fabrication process has been partly carried out at Waseda University Nanotechnology Research Center (NTRC). This work is partly supported by Japan Ministry of Education, Culture, Sports Science and Technology Grant-in-Aid for Scientific Basic Research (S) No. 23226010 of Waseda University, Global COE program “Ambient GCOE” of Waseda University.

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AB - A stand-alone microfluidic system consists of a reusable flow control part and a disposable microwell array for high throughput cell analysis is proposed. The flow control part is realized with addressable microvalve array driven by compactly-fabricated solenoid actuators and EOF micropumps. Disposable 16 microwell array with 4 reagent injection ports was fabricated using a PDMS structure. The disposable fluidic chip can be easily replaced to different functional fluidic networks depended on their actual uses. The prototype system with 2×5 (52) on-chip hydraulic valves that are driven by 10 (25) solenoid actuators was fabricated. Precise reagent injection of ten-nanoliter order to each microwell was successfully carried out.

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Stand-alone micro fluidic system using partly disposable PDMS microwell array for high throughput cell analysis

Abstract

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Keywords

ASJC Scopus subject areas

Access to Document

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