Stem cell separation using thermoresponsive copolymer brushes having cationic charge

Kenichi Nagase; Yuri Hatakeyama; Tatsuya Shimizu; Katsuhisa Matsuura; Masayuki Yamato; Naoya Takeda; Teruo Okano

doi:10.1109/MHS.2015.7438250

Stem cell separation using thermoresponsive copolymer brushes having cationic charge

Kenichi Nagase, Yuri Hatakeyama, Tatsuya Shimizu, Katsuhisa Matsuura, Masayuki Yamato, Naoya Takeda, Teruo Okano

School of Advanced Science and Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

For developing new stem cell separation tool, we have prepared thermoresponsive cationic copolymer brush, poly(N-isopropylacrylamide-co-N, N-dimethylaminopropylacrylamide-co-N-tert-butylacrylamide (IPAAm-co-DMAPAAm-co-tBAAm) on glass substrates through surface-initiated atom transfer radical copolymerization (ATRP). Using the prepared thermoresponsive cationic copolymer brush, mesenchymal stem cell separation was performed simply by changing temperature.

Original language	English
Title of host publication	2015 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2015
Publisher	Institute of Electrical and Electronics Engineers Inc.
ISBN (Electronic)	9781479966783
DOIs	https://doi.org/10.1109/MHS.2015.7438250
Publication status	Published - 2016 Mar 21
Event	International Symposium on Micro-NanoMechatronics and Human Science, MHS 2015 - Nagoya, Japan Duration: 2015 Nov 23 → 2015 Nov 25

Publication series

Name	2015 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2015

Other

Other	International Symposium on Micro-NanoMechatronics and Human Science, MHS 2015
Country/Territory	Japan
City	Nagoya
Period	15/11/23 → 15/11/25

ASJC Scopus subject areas

Biomedical Engineering
Electrical and Electronic Engineering
Biotechnology
Education

Access to Document

10.1109/MHS.2015.7438250

Cite this

Nagase, K., Hatakeyama, Y., Shimizu, T., Matsuura, K., Yamato, M., Takeda, N., & Okano, T. (2016). Stem cell separation using thermoresponsive copolymer brushes having cationic charge. In 2015 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2015 [7438250] (2015 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2015). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/MHS.2015.7438250

Stem cell separation using thermoresponsive copolymer brushes having cationic charge. / Nagase, Kenichi; Hatakeyama, Yuri; Shimizu, Tatsuya et al.

2015 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2015. Institute of Electrical and Electronics Engineers Inc., 2016. 7438250 (2015 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2015).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Nagase, K, Hatakeyama, Y, Shimizu, T, Matsuura, K, Yamato, M, Takeda, N & Okano, T 2016, Stem cell separation using thermoresponsive copolymer brushes having cationic charge. in 2015 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2015., 7438250, 2015 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2015, Institute of Electrical and Electronics Engineers Inc., International Symposium on Micro-NanoMechatronics and Human Science, MHS 2015, Nagoya, Japan, 15/11/23. https://doi.org/10.1109/MHS.2015.7438250

Nagase K, Hatakeyama Y, Shimizu T, Matsuura K, Yamato M, Takeda N et al. Stem cell separation using thermoresponsive copolymer brushes having cationic charge. In 2015 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2015. Institute of Electrical and Electronics Engineers Inc. 2016. 7438250. (2015 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2015). doi: 10.1109/MHS.2015.7438250

Nagase, Kenichi ; Hatakeyama, Yuri ; Shimizu, Tatsuya et al. / Stem cell separation using thermoresponsive copolymer brushes having cationic charge. 2015 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2015. Institute of Electrical and Electronics Engineers Inc., 2016. (2015 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2015).

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AU - Takeda, Naoya

AU - Okano, Teruo

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AB - For developing new stem cell separation tool, we have prepared thermoresponsive cationic copolymer brush, poly(N-isopropylacrylamide-co-N, N-dimethylaminopropylacrylamide-co-N-tert-butylacrylamide (IPAAm-co-DMAPAAm-co-tBAAm) on glass substrates through surface-initiated atom transfer radical copolymerization (ATRP). Using the prepared thermoresponsive cationic copolymer brush, mesenchymal stem cell separation was performed simply by changing temperature.

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Stem cell separation using thermoresponsive copolymer brushes having cationic charge

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