Stepwise pattern modification of neuronal network during cultivation using photo-thermal etching of agarose architecture

Yoshihiro Sugio, Hiroyuki Moriguchi, Ikurou Suzuki, Tomoyuki Kaneko, Kenji Yasuda, Yasuhiko Jimbo

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We have developed a new type of on-chip cell cultivation system using an agarose microchamber (AMC) array and a photo-thermal etching method, thus enabling topographical control of neuronal network pattern step-by-step during cell cultivation. By using photo-thermal etching (micro melting) method, the number of microtunnel connecting microchambers can be easily increased, even during cell cultivation, according to the progress of the neuronal network formation. To demonstrate the capability of this system for topographical control of network formation, we cultured hippocampal neurons in this AMC array. We found that the cells in microchambers made fiber connections through microtunnels. Furthermore the cells even made fiber connections through additional microtunnels fabricated during cultivation by photo-thermal etching. The results showed that the photo-thermal etching could be used during cultivation without damaging cells.

Original languageEnglish
Title of host publicationDigest of Papers - Microprocesses and Nanotechnology 2003 - 2003 International Microprocesses and Nanotechnology Conference, MNC 2003
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages322-323
Number of pages2
ISBN (Electronic)4891140402, 9784891140403
DOIs
Publication statusPublished - 2003
Externally publishedYes
EventInternational Microprocesses and Nanotechnology Conference, MNC 2003 - Tokyo, Japan
Duration: 2003 Oct 292003 Oct 31

Other

OtherInternational Microprocesses and Nanotechnology Conference, MNC 2003
CountryJapan
CityTokyo
Period03/10/2903/10/31

Fingerprint

Etching
Fibers
Neurons
Melting
Hot Temperature

ASJC Scopus subject areas

  • Hardware and Architecture
  • Electrical and Electronic Engineering

Cite this

Sugio, Y., Moriguchi, H., Suzuki, I., Kaneko, T., Yasuda, K., & Jimbo, Y. (2003). Stepwise pattern modification of neuronal network during cultivation using photo-thermal etching of agarose architecture. In Digest of Papers - Microprocesses and Nanotechnology 2003 - 2003 International Microprocesses and Nanotechnology Conference, MNC 2003 (pp. 322-323). [1268776] Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/IMNC.2003.1268776

Stepwise pattern modification of neuronal network during cultivation using photo-thermal etching of agarose architecture. / Sugio, Yoshihiro; Moriguchi, Hiroyuki; Suzuki, Ikurou; Kaneko, Tomoyuki; Yasuda, Kenji; Jimbo, Yasuhiko.

Digest of Papers - Microprocesses and Nanotechnology 2003 - 2003 International Microprocesses and Nanotechnology Conference, MNC 2003. Institute of Electrical and Electronics Engineers Inc., 2003. p. 322-323 1268776.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Sugio, Y, Moriguchi, H, Suzuki, I, Kaneko, T, Yasuda, K & Jimbo, Y 2003, Stepwise pattern modification of neuronal network during cultivation using photo-thermal etching of agarose architecture. in Digest of Papers - Microprocesses and Nanotechnology 2003 - 2003 International Microprocesses and Nanotechnology Conference, MNC 2003., 1268776, Institute of Electrical and Electronics Engineers Inc., pp. 322-323, International Microprocesses and Nanotechnology Conference, MNC 2003, Tokyo, Japan, 03/10/29. https://doi.org/10.1109/IMNC.2003.1268776
Sugio Y, Moriguchi H, Suzuki I, Kaneko T, Yasuda K, Jimbo Y. Stepwise pattern modification of neuronal network during cultivation using photo-thermal etching of agarose architecture. In Digest of Papers - Microprocesses and Nanotechnology 2003 - 2003 International Microprocesses and Nanotechnology Conference, MNC 2003. Institute of Electrical and Electronics Engineers Inc. 2003. p. 322-323. 1268776 https://doi.org/10.1109/IMNC.2003.1268776
Sugio, Yoshihiro ; Moriguchi, Hiroyuki ; Suzuki, Ikurou ; Kaneko, Tomoyuki ; Yasuda, Kenji ; Jimbo, Yasuhiko. / Stepwise pattern modification of neuronal network during cultivation using photo-thermal etching of agarose architecture. Digest of Papers - Microprocesses and Nanotechnology 2003 - 2003 International Microprocesses and Nanotechnology Conference, MNC 2003. Institute of Electrical and Electronics Engineers Inc., 2003. pp. 322-323
@inproceedings{68e1352cef7f488eab704e893e19f844,
title = "Stepwise pattern modification of neuronal network during cultivation using photo-thermal etching of agarose architecture",
abstract = "We have developed a new type of on-chip cell cultivation system using an agarose microchamber (AMC) array and a photo-thermal etching method, thus enabling topographical control of neuronal network pattern step-by-step during cell cultivation. By using photo-thermal etching (micro melting) method, the number of microtunnel connecting microchambers can be easily increased, even during cell cultivation, according to the progress of the neuronal network formation. To demonstrate the capability of this system for topographical control of network formation, we cultured hippocampal neurons in this AMC array. We found that the cells in microchambers made fiber connections through microtunnels. Furthermore the cells even made fiber connections through additional microtunnels fabricated during cultivation by photo-thermal etching. The results showed that the photo-thermal etching could be used during cultivation without damaging cells.",
author = "Yoshihiro Sugio and Hiroyuki Moriguchi and Ikurou Suzuki and Tomoyuki Kaneko and Kenji Yasuda and Yasuhiko Jimbo",
year = "2003",
doi = "10.1109/IMNC.2003.1268776",
language = "English",
pages = "322--323",
booktitle = "Digest of Papers - Microprocesses and Nanotechnology 2003 - 2003 International Microprocesses and Nanotechnology Conference, MNC 2003",
publisher = "Institute of Electrical and Electronics Engineers Inc.",
address = "United States",

}

TY - GEN

T1 - Stepwise pattern modification of neuronal network during cultivation using photo-thermal etching of agarose architecture

AU - Sugio, Yoshihiro

AU - Moriguchi, Hiroyuki

AU - Suzuki, Ikurou

AU - Kaneko, Tomoyuki

AU - Yasuda, Kenji

AU - Jimbo, Yasuhiko

PY - 2003

Y1 - 2003

N2 - We have developed a new type of on-chip cell cultivation system using an agarose microchamber (AMC) array and a photo-thermal etching method, thus enabling topographical control of neuronal network pattern step-by-step during cell cultivation. By using photo-thermal etching (micro melting) method, the number of microtunnel connecting microchambers can be easily increased, even during cell cultivation, according to the progress of the neuronal network formation. To demonstrate the capability of this system for topographical control of network formation, we cultured hippocampal neurons in this AMC array. We found that the cells in microchambers made fiber connections through microtunnels. Furthermore the cells even made fiber connections through additional microtunnels fabricated during cultivation by photo-thermal etching. The results showed that the photo-thermal etching could be used during cultivation without damaging cells.

AB - We have developed a new type of on-chip cell cultivation system using an agarose microchamber (AMC) array and a photo-thermal etching method, thus enabling topographical control of neuronal network pattern step-by-step during cell cultivation. By using photo-thermal etching (micro melting) method, the number of microtunnel connecting microchambers can be easily increased, even during cell cultivation, according to the progress of the neuronal network formation. To demonstrate the capability of this system for topographical control of network formation, we cultured hippocampal neurons in this AMC array. We found that the cells in microchambers made fiber connections through microtunnels. Furthermore the cells even made fiber connections through additional microtunnels fabricated during cultivation by photo-thermal etching. The results showed that the photo-thermal etching could be used during cultivation without damaging cells.

UR - http://www.scopus.com/inward/record.url?scp=84949211503&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84949211503&partnerID=8YFLogxK

U2 - 10.1109/IMNC.2003.1268776

DO - 10.1109/IMNC.2003.1268776

M3 - Conference contribution

AN - SCOPUS:84949211503

SP - 322

EP - 323

BT - Digest of Papers - Microprocesses and Nanotechnology 2003 - 2003 International Microprocesses and Nanotechnology Conference, MNC 2003

PB - Institute of Electrical and Electronics Engineers Inc.

ER -