Stepwise pattern modification of neuronal network during cultivation using photo-thermal etching of agarose architecture

Yoshihiro Sugio; Hiroyuki Moriguchi; Ikurou Suzuki; Tomoyuki Kaneko; Kenji Yasuda; Yasuhiko Jimbo

doi:10.1109/IMNC.2003.1268776

Stepwise pattern modification of neuronal network during cultivation using photo-thermal etching of agarose architecture

Yoshihiro Sugio, Hiroyuki Moriguchi, Ikurou Suzuki, Tomoyuki Kaneko, Kenji Yasuda, Yasuhiko Jimbo

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

We have developed a new type of on-chip cell cultivation system using an agarose microchamber (AMC) array and a photo-thermal etching method, thus enabling topographical control of neuronal network pattern step-by-step during cell cultivation. By using photo-thermal etching (micro melting) method, the number of microtunnel connecting microchambers can be easily increased, even during cell cultivation, according to the progress of the neuronal network formation. To demonstrate the capability of this system for topographical control of network formation, we cultured hippocampal neurons in this AMC array. We found that the cells in microchambers made fiber connections through microtunnels. Furthermore the cells even made fiber connections through additional microtunnels fabricated during cultivation by photo-thermal etching. The results showed that the photo-thermal etching could be used during cultivation without damaging cells.

Original language	English
Title of host publication	Digest of Papers - Microprocesses and Nanotechnology 2003 - 2003 International Microprocesses and Nanotechnology Conference, MNC 2003
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	322-323
Number of pages	2
ISBN (Electronic)	4891140402, 9784891140403
DOIs	https://doi.org/10.1109/IMNC.2003.1268776
Publication status	Published - 2003
Externally published	Yes
Event	International Microprocesses and Nanotechnology Conference, MNC 2003 - Tokyo, Japan Duration: 2003 Oct 29 → 2003 Oct 31

Other

Other	International Microprocesses and Nanotechnology Conference, MNC 2003
Country/Territory	Japan
City	Tokyo
Period	03/10/29 → 03/10/31

ASJC Scopus subject areas

Hardware and Architecture
Electrical and Electronic Engineering

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10.1109/IMNC.2003.1268776

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Sugio, Y., Moriguchi, H., Suzuki, I., Kaneko, T., Yasuda, K., & Jimbo, Y. (2003). Stepwise pattern modification of neuronal network during cultivation using photo-thermal etching of agarose architecture. In Digest of Papers - Microprocesses and Nanotechnology 2003 - 2003 International Microprocesses and Nanotechnology Conference, MNC 2003 (pp. 322-323). [1268776] Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/IMNC.2003.1268776

Stepwise pattern modification of neuronal network during cultivation using photo-thermal etching of agarose architecture. / Sugio, Yoshihiro; Moriguchi, Hiroyuki; Suzuki, Ikurou et al.

Digest of Papers - Microprocesses and Nanotechnology 2003 - 2003 International Microprocesses and Nanotechnology Conference, MNC 2003. Institute of Electrical and Electronics Engineers Inc., 2003. p. 322-323 1268776.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Sugio, Y, Moriguchi, H, Suzuki, I, Kaneko, T, Yasuda, K & Jimbo, Y 2003, Stepwise pattern modification of neuronal network during cultivation using photo-thermal etching of agarose architecture. in Digest of Papers - Microprocesses and Nanotechnology 2003 - 2003 International Microprocesses and Nanotechnology Conference, MNC 2003., 1268776, Institute of Electrical and Electronics Engineers Inc., pp. 322-323, International Microprocesses and Nanotechnology Conference, MNC 2003, Tokyo, Japan, 03/10/29. https://doi.org/10.1109/IMNC.2003.1268776

Sugio Y, Moriguchi H, Suzuki I, Kaneko T, Yasuda K, Jimbo Y. Stepwise pattern modification of neuronal network during cultivation using photo-thermal etching of agarose architecture. In Digest of Papers - Microprocesses and Nanotechnology 2003 - 2003 International Microprocesses and Nanotechnology Conference, MNC 2003. Institute of Electrical and Electronics Engineers Inc. 2003. p. 322-323. 1268776 doi: 10.1109/IMNC.2003.1268776

Sugio, Yoshihiro ; Moriguchi, Hiroyuki ; Suzuki, Ikurou et al. / Stepwise pattern modification of neuronal network during cultivation using photo-thermal etching of agarose architecture. Digest of Papers - Microprocesses and Nanotechnology 2003 - 2003 International Microprocesses and Nanotechnology Conference, MNC 2003. Institute of Electrical and Electronics Engineers Inc., 2003. pp. 322-323

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title = "Stepwise pattern modification of neuronal network during cultivation using photo-thermal etching of agarose architecture",

abstract = "We have developed a new type of on-chip cell cultivation system using an agarose microchamber (AMC) array and a photo-thermal etching method, thus enabling topographical control of neuronal network pattern step-by-step during cell cultivation. By using photo-thermal etching (micro melting) method, the number of microtunnel connecting microchambers can be easily increased, even during cell cultivation, according to the progress of the neuronal network formation. To demonstrate the capability of this system for topographical control of network formation, we cultured hippocampal neurons in this AMC array. We found that the cells in microchambers made fiber connections through microtunnels. Furthermore the cells even made fiber connections through additional microtunnels fabricated during cultivation by photo-thermal etching. The results showed that the photo-thermal etching could be used during cultivation without damaging cells.",

author = "Yoshihiro Sugio and Hiroyuki Moriguchi and Ikurou Suzuki and Tomoyuki Kaneko and Kenji Yasuda and Yasuhiko Jimbo",

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AU - Yasuda, Kenji

AU - Jimbo, Yasuhiko

PY - 2003

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AB - We have developed a new type of on-chip cell cultivation system using an agarose microchamber (AMC) array and a photo-thermal etching method, thus enabling topographical control of neuronal network pattern step-by-step during cell cultivation. By using photo-thermal etching (micro melting) method, the number of microtunnel connecting microchambers can be easily increased, even during cell cultivation, according to the progress of the neuronal network formation. To demonstrate the capability of this system for topographical control of network formation, we cultured hippocampal neurons in this AMC array. We found that the cells in microchambers made fiber connections through microtunnels. Furthermore the cells even made fiber connections through additional microtunnels fabricated during cultivation by photo-thermal etching. The results showed that the photo-thermal etching could be used during cultivation without damaging cells.

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Stepwise pattern modification of neuronal network during cultivation using photo-thermal etching of agarose architecture

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