Stimulation of citric acid production in Aspergillus niger by addition of viscous substances in shake culture

S. Rugasaseel, S. Morikawa, Kotaro Kirimura, S. Usami

    Research output: Contribution to journalArticle

    13 Citations (Scopus)

    Abstract

    When glucose (120mg/ml) was used as a carbon source, Aspergillus niger Yang no. 2. showed a markedly low citric acid productivity in shake culture (15.4 mg/ml) but a high productivity in semi-solid and surface cultures (72.3 mg/ml and 67.6 mg/ml, respectively). Since the viscosity of the medium was assumed to be one of the important factors for citric acid productivity in shake culture, the effects of the addition of viscous substances on citric acid productivity of strain Yang no. 2 were examined. The addition of 2.0-6.0 mg gelatin/ml as a viscous additive to the medium containing glucose as a carbon source increased slightly the medium viscosity but substantially increased the citric acid productivity in shake culture to levels of 52.0-53.3 mg/ml, about 3.4 times as much as that without gelatin. However, no influence of gelatin addition was observed in semi-solid and surface cultures, i.e. under static cultivation conditions. Different mycelial morphologies of the strain were observed when cultivations were done in shake culture with or without the addition of gelatin. Addition of 5.0 mg agar/ml, 5.0 mg carageenan/ml, 2.5 mg carboxymethylcellulose/ml and 2.5 mg polyethylene glycol 6000/ml, to the medium containing glucose as a carbon source also increased the citric acid productivity in shake culture to levels of 39.2-54.7 mg/ml. Since Yang no. 2 does not utilize these viscous substances, these results suggested that the viscous substances functioned as protectants for the mycelium from physiological stresses due to shaking and as a consequence resulted in a remarkably increased citric acid productivity in shake culture.

    Original languageEnglish
    Pages (from-to)839-843
    Number of pages5
    JournalApplied Microbiology and Biotechnology
    Volume42
    Issue number6
    DOIs
    Publication statusPublished - 1995 Mar

    Fingerprint

    Aspergillus niger
    Aspergillus
    Citric acid
    Citric Acid
    Productivity
    Gelatin
    Carbon
    Glucose
    Viscosity
    Carboxymethylcellulose Sodium
    Physiological Stress
    Mycelium
    Agar
    Polyethylene glycols

    ASJC Scopus subject areas

    • Microbiology (medical)
    • Microbiology
    • Bioengineering
    • Biotechnology

    Cite this

    Stimulation of citric acid production in Aspergillus niger by addition of viscous substances in shake culture. / Rugasaseel, S.; Morikawa, S.; Kirimura, Kotaro; Usami, S.

    In: Applied Microbiology and Biotechnology, Vol. 42, No. 6, 03.1995, p. 839-843.

    Research output: Contribution to journalArticle

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