Stimulation of Dmc1-mediated DNA strand exchange by the human Rad54B protein

Naoyuki Sarai, Wataru Kagawa, Takashi Kinebuchi, Ako Kagawa, Kozo Tanaka, Kiyoshi Miyagawa, Shukuko Ikawa, Takehiko Shibata, Hitoshi Kurumizaka*, Shigeyuki Yokoyama

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    16 Citations (Scopus)

    Abstract

    The process of homologous recombination is indispensable for both meiotic and mitotic cell division, and is one of the major pathways for double-strand break (DSB) repair. The human Rad54B protein, which belongs to the SWI2/SNF2 protein family, plays a role in homologous recombination, and may function with the Dmc1 recombinase, a meiosis-specific Rad51 homolog. In the present study, we found that Rad54B enhanced the DNA strand-exchange activity of Dmc1 by stabilizing the Dmc1-single-stranded DNA (ssDNA) complex. Therefore, Rad54B may stimulate the Dmc1-mediated DNA strand exchange by stabilizing the nucleoprotein filament, which is formed on the ssDNA tails produced at DSB sites during homologous recombination.

    Original languageEnglish
    Pages (from-to)4429-4437
    Number of pages9
    JournalNucleic Acids Research
    Volume34
    Issue number16
    DOIs
    Publication statusPublished - 2006 Sep

    ASJC Scopus subject areas

    • Genetics

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