Stimulation of protein synthesis in pachytene primary spermatocytes from the human testis by pyruvate

M. Nakamura, K. Ishida, M. Waku

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Abstract

A sequential enzymatic incubation in collagenase and trypsin was carried out to yield a suspension of viable single cells from the seminiferous epithelium of adult human testis. The cell suspension predominantly consisted of pachytene primary spermatocytes (15%), round spermatids (32%), and condensing spermatids and residual bodies (21%). Human pachytene spermatocytes were isolated by unit gravity sedimentation using the methods originally developed for murine tissue. The spermatocyte-enriched fraction was 79% pure. When the effect of energy sources on protein synthesis by spermatocytes was examined, the highest rate of protein synthesis with pyruvate was found among four kinds of substrates added at a concentration of 10 mM. As shown with murine spermatocytes, the rate of protein synthesis by the human spermatocytes is probably regulated by pyruvate.

Original languageEnglish
Pages (from-to)461-465
Number of pages5
JournalAndrologia
Volume17
Issue number5
Publication statusPublished - 1985
Externally publishedYes

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ASJC Scopus subject areas

  • Endocrinology
  • Nephrology

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