Stimulation of protein synthesis in round spermatids from rat testes by lactate

Masahisa Nakamura, Akiya Hino, Ikuo Yasumasu, Junzo Kato

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Lactate markedly increased the rate of [3H]leucine incorporation into the protein of isolated round spermatids (steps 1-8) from rat testes. Four kinds of hexoses, glucose, fructose, galactose, and monnose, also stimulated [3H]leucine incorporation, but to much lesser extents than lactate. Ribose had no effect. The glucose-induced stimulation of protein synthesis was entirely suppressed by iodoacetate and NaF, whereas iodoacetate and NaF were without effect on the lactate-induced increase in protein synthesis. Lactate stimulated both protein synthesis and ATP production in the spermatids. However, both of these stimulatory effects of lactate were completely blocked by DNP and rotenone. Rotenone entirely blocked oxygen consumption, as expected, whilst DNP enhanced it additively with lactate. Moreover, lactate was without influence on either transport of α-[3H]AIB into spermatids or incorporation of [3H]Ieucine into protein of a cell-free system of spermatids.These findings suggest that lactate may increase the protein synthesis of spermatids in the same fashion as glucose, and that the effect of lactate in increasing the level of ATP during incubation in vitro may be a major factor in the mechanism of stimulation of protein synthesis in the spermatids.

Original languageEnglish
Pages (from-to)1309-1315
Number of pages7
JournalJournal of Biochemistry
Volume89
Issue number4
Publication statusPublished - 1981 Apr
Externally publishedYes

Fingerprint

Spermatids
Protein Synthesis
Testis
Rats
Lactic Acid
Proteins
Glucose
Adenosinetriphosphate
Iodoacetates
Rotenone
Protein
Oxygen Consumption
Leucine
Fructose
Adenosine Triphosphate
Rat
Hexoses
Ribose
Cell-Free System
Cell

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Radiology Nuclear Medicine and imaging
  • Molecular Biology
  • Biochemistry

Cite this

Nakamura, M., Hino, A., Yasumasu, I., & Kato, J. (1981). Stimulation of protein synthesis in round spermatids from rat testes by lactate. Journal of Biochemistry, 89(4), 1309-1315.

Stimulation of protein synthesis in round spermatids from rat testes by lactate. / Nakamura, Masahisa; Hino, Akiya; Yasumasu, Ikuo; Kato, Junzo.

In: Journal of Biochemistry, Vol. 89, No. 4, 04.1981, p. 1309-1315.

Research output: Contribution to journalArticle

Nakamura, M, Hino, A, Yasumasu, I & Kato, J 1981, 'Stimulation of protein synthesis in round spermatids from rat testes by lactate', Journal of Biochemistry, vol. 89, no. 4, pp. 1309-1315.
Nakamura M, Hino A, Yasumasu I, Kato J. Stimulation of protein synthesis in round spermatids from rat testes by lactate. Journal of Biochemistry. 1981 Apr;89(4):1309-1315.
Nakamura, Masahisa ; Hino, Akiya ; Yasumasu, Ikuo ; Kato, Junzo. / Stimulation of protein synthesis in round spermatids from rat testes by lactate. In: Journal of Biochemistry. 1981 ; Vol. 89, No. 4. pp. 1309-1315.
@article{eebc9a787974439f966f26c9c3f98adf,
title = "Stimulation of protein synthesis in round spermatids from rat testes by lactate",
abstract = "Lactate markedly increased the rate of [3H]leucine incorporation into the protein of isolated round spermatids (steps 1-8) from rat testes. Four kinds of hexoses, glucose, fructose, galactose, and monnose, also stimulated [3H]leucine incorporation, but to much lesser extents than lactate. Ribose had no effect. The glucose-induced stimulation of protein synthesis was entirely suppressed by iodoacetate and NaF, whereas iodoacetate and NaF were without effect on the lactate-induced increase in protein synthesis. Lactate stimulated both protein synthesis and ATP production in the spermatids. However, both of these stimulatory effects of lactate were completely blocked by DNP and rotenone. Rotenone entirely blocked oxygen consumption, as expected, whilst DNP enhanced it additively with lactate. Moreover, lactate was without influence on either transport of α-[3H]AIB into spermatids or incorporation of [3H]Ieucine into protein of a cell-free system of spermatids.These findings suggest that lactate may increase the protein synthesis of spermatids in the same fashion as glucose, and that the effect of lactate in increasing the level of ATP during incubation in vitro may be a major factor in the mechanism of stimulation of protein synthesis in the spermatids.",
author = "Masahisa Nakamura and Akiya Hino and Ikuo Yasumasu and Junzo Kato",
year = "1981",
month = "4",
language = "English",
volume = "89",
pages = "1309--1315",
journal = "Journal of Biochemistry",
issn = "0021-924X",
publisher = "Oxford University Press",
number = "4",

}

TY - JOUR

T1 - Stimulation of protein synthesis in round spermatids from rat testes by lactate

AU - Nakamura, Masahisa

AU - Hino, Akiya

AU - Yasumasu, Ikuo

AU - Kato, Junzo

PY - 1981/4

Y1 - 1981/4

N2 - Lactate markedly increased the rate of [3H]leucine incorporation into the protein of isolated round spermatids (steps 1-8) from rat testes. Four kinds of hexoses, glucose, fructose, galactose, and monnose, also stimulated [3H]leucine incorporation, but to much lesser extents than lactate. Ribose had no effect. The glucose-induced stimulation of protein synthesis was entirely suppressed by iodoacetate and NaF, whereas iodoacetate and NaF were without effect on the lactate-induced increase in protein synthesis. Lactate stimulated both protein synthesis and ATP production in the spermatids. However, both of these stimulatory effects of lactate were completely blocked by DNP and rotenone. Rotenone entirely blocked oxygen consumption, as expected, whilst DNP enhanced it additively with lactate. Moreover, lactate was without influence on either transport of α-[3H]AIB into spermatids or incorporation of [3H]Ieucine into protein of a cell-free system of spermatids.These findings suggest that lactate may increase the protein synthesis of spermatids in the same fashion as glucose, and that the effect of lactate in increasing the level of ATP during incubation in vitro may be a major factor in the mechanism of stimulation of protein synthesis in the spermatids.

AB - Lactate markedly increased the rate of [3H]leucine incorporation into the protein of isolated round spermatids (steps 1-8) from rat testes. Four kinds of hexoses, glucose, fructose, galactose, and monnose, also stimulated [3H]leucine incorporation, but to much lesser extents than lactate. Ribose had no effect. The glucose-induced stimulation of protein synthesis was entirely suppressed by iodoacetate and NaF, whereas iodoacetate and NaF were without effect on the lactate-induced increase in protein synthesis. Lactate stimulated both protein synthesis and ATP production in the spermatids. However, both of these stimulatory effects of lactate were completely blocked by DNP and rotenone. Rotenone entirely blocked oxygen consumption, as expected, whilst DNP enhanced it additively with lactate. Moreover, lactate was without influence on either transport of α-[3H]AIB into spermatids or incorporation of [3H]Ieucine into protein of a cell-free system of spermatids.These findings suggest that lactate may increase the protein synthesis of spermatids in the same fashion as glucose, and that the effect of lactate in increasing the level of ATP during incubation in vitro may be a major factor in the mechanism of stimulation of protein synthesis in the spermatids.

UR - http://www.scopus.com/inward/record.url?scp=0019455072&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019455072&partnerID=8YFLogxK

M3 - Article

VL - 89

SP - 1309

EP - 1315

JO - Journal of Biochemistry

JF - Journal of Biochemistry

SN - 0021-924X

IS - 4

ER -