Stimulation of Spermatid Phosphofructokinase by Fructose 2,6‐Bisphosphate from Rat Testes: fructose 2, 6‐bisphosphate/phosphofructokinase/spermatids/rats

MASAHISA NAKAMURA, AKIRA SUZUKI, SHOICHI OKINAGA, KIYOSHI ARAI

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Effect of fructose 2, 6‐bisphosphate on 6‐phosphofructokinase (ATP: D‐fructose‐6‐phosphate 1‐phosphotransferase, EC 2.7.1.11) in spermatid extract from rat testes was studied. Fructose 2, 6‐bisphosphate stimulated the enzyme greatly by increasing its affinity for fructose 6‐phosphate and relieving the inhibition by ATP. Fructose 2, 6‐bisphosphate (0.8 μM) was required for 50% activation of 6‐phosphofructokinase (PFK). In addition, fructose 2, 6‐bisphosphate, AMP and fructose 6‐phosphate acted cooperatively to stimulate the activity of PFK. This stimulation may play an important role in the regulation of glycolysis in spermatids of rat testes.

Original languageEnglish
Pages (from-to)11-16
Number of pages6
JournalDevelopment Growth and Differentiation
Volume26
Issue number1
DOIs
Publication statusPublished - 1984
Externally publishedYes

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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