Stimulation of Spermatid Phosphofructokinase by Fructose 2,6‐Bisphosphate from Rat Testes: fructose 2, 6‐bisphosphate/phosphofructokinase/spermatids/rats

MASAHISA NAKAMURA, AKIRA SUZUKI, SHOICHI OKINAGA, KIYOSHI ARAI

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Effect of fructose 2, 6‐bisphosphate on 6‐phosphofructokinase (ATP: D‐fructose‐6‐phosphate 1‐phosphotransferase, EC 2.7.1.11) in spermatid extract from rat testes was studied. Fructose 2, 6‐bisphosphate stimulated the enzyme greatly by increasing its affinity for fructose 6‐phosphate and relieving the inhibition by ATP. Fructose 2, 6‐bisphosphate (0.8 μM) was required for 50% activation of 6‐phosphofructokinase (PFK). In addition, fructose 2, 6‐bisphosphate, AMP and fructose 6‐phosphate acted cooperatively to stimulate the activity of PFK. This stimulation may play an important role in the regulation of glycolysis in spermatids of rat testes.

Original languageEnglish
Pages (from-to)11-16
Number of pages6
JournalDevelopment Growth and Differentiation
Volume26
Issue number1
DOIs
Publication statusPublished - 1984
Externally publishedYes

Fingerprint

Phosphofructokinase-2
Spermatids
Fructose
Testis
Adenosine Triphosphate
Glycolysis
Adenosine Monophosphate
Enzymes

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Cite this

Stimulation of Spermatid Phosphofructokinase by Fructose 2,6‐Bisphosphate from Rat Testes : fructose 2, 6‐bisphosphate/phosphofructokinase/spermatids/rats. / NAKAMURA, MASAHISA; SUZUKI, AKIRA; OKINAGA, SHOICHI; ARAI, KIYOSHI.

In: Development Growth and Differentiation, Vol. 26, No. 1, 1984, p. 11-16.

Research output: Contribution to journalArticle

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abstract = "Effect of fructose 2, 6‐bisphosphate on 6‐phosphofructokinase (ATP: D‐fructose‐6‐phosphate 1‐phosphotransferase, EC 2.7.1.11) in spermatid extract from rat testes was studied. Fructose 2, 6‐bisphosphate stimulated the enzyme greatly by increasing its affinity for fructose 6‐phosphate and relieving the inhibition by ATP. Fructose 2, 6‐bisphosphate (0.8 μM) was required for 50{\%} activation of 6‐phosphofructokinase (PFK). In addition, fructose 2, 6‐bisphosphate, AMP and fructose 6‐phosphate acted cooperatively to stimulate the activity of PFK. This stimulation may play an important role in the regulation of glycolysis in spermatids of rat testes.",
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