Stoichiometry of the reaction of oxyhemoglobin with nitrite

Hiroaki Kosaka, Kazuhiko Imaizumi, Kiyohiro Imai, Itiro Tyuma

Research output: Contribution to journalArticle

110 Citations (Scopus)

Abstract

During the reaction of oxyhemoglobin (HbO2) with nitrite, the concentration of residual nitrite, nitrate, oxygen, and methemoglobin (Hb+) was determined successively. The results obtained at various pH values indicate the following stoichiometry for the overall reaction: 4HbO2 + 4NO- 2 + → 4Hb+ + 4NO- 3 + O2 + 2H2O (Hb denotes hemoglobin monomer). NO2 - binds with methemoglobin noncooperatively with a binding constant of 340 M-1 at pH 7.4 and 25°C. Thus, the major part of Hb+ produced is aquomethemoglobin, not methemoglobin nitrite, when less than 2 equivalents of nitrite is used for the oxidation.

Original languageEnglish
Pages (from-to)184-188
Number of pages5
JournalBBA - Protein Structure
Volume581
Issue number1
DOIs
Publication statusPublished - 1979 Nov 23
Externally publishedYes

Fingerprint

Oxyhemoglobins
Nitrites
Stoichiometry
Methemoglobin
Nitrates
Hemoglobins
Monomers
Oxygen
Oxidation

Keywords

  • Nitrite
  • Oxyhemoglobin
  • Stoichiometry

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Stoichiometry of the reaction of oxyhemoglobin with nitrite. / Kosaka, Hiroaki; Imaizumi, Kazuhiko; Imai, Kiyohiro; Tyuma, Itiro.

In: BBA - Protein Structure, Vol. 581, No. 1, 23.11.1979, p. 184-188.

Research output: Contribution to journalArticle

Kosaka, H, Imaizumi, K, Imai, K & Tyuma, I 1979, 'Stoichiometry of the reaction of oxyhemoglobin with nitrite', BBA - Protein Structure, vol. 581, no. 1, pp. 184-188. https://doi.org/10.1016/0005-2795(79)90235-6
Kosaka, Hiroaki ; Imaizumi, Kazuhiko ; Imai, Kiyohiro ; Tyuma, Itiro. / Stoichiometry of the reaction of oxyhemoglobin with nitrite. In: BBA - Protein Structure. 1979 ; Vol. 581, No. 1. pp. 184-188.
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