Stress increases putative gonadotropin inhibitory hormone and decreases luteinizing hormone in male rats

Elizabeth D. Kirby, Anna C. Geraghty, Takayoshi Ubuka, George E. Bentley, Daniela Kaufer

    Research output: Contribution to journalArticle

    209 Citations (Scopus)

    Abstract

    The subjective experience of stress leads to reproductive dysfunction in many species, including rodents and humans. Stress effects on reproduction result from multilevel interactions between the hormonal stress response system, i.e., the hypothalamic-pituitary-adrenal (HPA) axis, and the hormonal reproductive system, i.e., the hypothalamic-pituitary-gonadal (HPG) axis. A novel negative regulator of the HPG axis known as gonadotropin-inhibitory hormone (GnIH) was recently discovered in quail, and orthologous neuropeptides known as RFamide-related peptides (RFRPs) have also been identified in rodents and primates. It is currently unknown, however, whether GnIH/RFRPs influence HPG axis activity in response to stress. We show here that both acute and chronic immobilization stress lead to an up-regulation of RFRP expression in the dorsomedial hypothalamus (DMH) of adult male rats and that this increase in RFRP is associated with inhibition of downstream HPG activity.Wealso show that adrenalectomy blocks the stress-induced increase in RFRP expression. Immunohistochemistry revealed that 53% of RFRP cells express receptors for glucocorticoids (GCs), indicating that adrenal GCs can mediate the stress effect through direct action on RFRP cells. It is thought that stress effects on central control of reproduction are largely mediated by direct or indirect effects on GnRH-secreting neurons. Our data show that stress-induced increases in adrenal GCs cause an increase in RFRP that contributes to hypothalamic suppression of reproductive function. This novel insight into HPA-HPG interaction provides a paradigm shift for work on stress-related reproductive dysfunction and infertility, and indicates that future work on stress and reproductive system interactions must include investigation of the role of GnIH/RFRP.

    Original languageEnglish
    Pages (from-to)11324-11329
    Number of pages6
    JournalProceedings of the National Academy of Sciences of the United States of America
    Volume106
    Issue number27
    DOIs
    Publication statusPublished - 2009 Jul 7

    Fingerprint

    Luteinizing Hormone
    Gonadotropins
    Hormones
    Glucocorticoids
    Reproduction
    Rodentia
    RFamide peptide
    Quail
    Adrenalectomy
    Glucocorticoid Receptors
    Neuropeptides
    Gonadotropin-Releasing Hormone
    Immobilization
    Infertility
    Primates
    Hypothalamus
    Up-Regulation
    Immunohistochemistry
    Neurons

    Keywords

    • GnIH
    • Reproduction
    • RFRP

    ASJC Scopus subject areas

    • General

    Cite this

    Stress increases putative gonadotropin inhibitory hormone and decreases luteinizing hormone in male rats. / Kirby, Elizabeth D.; Geraghty, Anna C.; Ubuka, Takayoshi; Bentley, George E.; Kaufer, Daniela.

    In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, No. 27, 07.07.2009, p. 11324-11329.

    Research output: Contribution to journalArticle

    Kirby, Elizabeth D. ; Geraghty, Anna C. ; Ubuka, Takayoshi ; Bentley, George E. ; Kaufer, Daniela. / Stress increases putative gonadotropin inhibitory hormone and decreases luteinizing hormone in male rats. In: Proceedings of the National Academy of Sciences of the United States of America. 2009 ; Vol. 106, No. 27. pp. 11324-11329.
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