Stress relaxation and stress-strain characteristics of porcine amniotic membrane

Masato Kikuchi, Zhonggang Feng, Tadashi Kosawada, Daisuke Sato, Takao Nakamura, Mitsuo Umezu

    Research output: Contribution to journalArticle

    3 Citations (Scopus)

    Abstract

    BACKGROUND: Recently, amniotic membrane (AM) as scaffold is accumulating much more attention in tissue engineering. It is well-known that the mechanical properties of the scaffold inevitably affect the biological process of the incorporated cells. OBJECTIVE: This study investigates the stress relaxation and stress-strain characteristics of AM, which have not been sufficiently elucidated before. METHODS: Porcine AMsamples were prepared at four different AMregions and at three different directions. Ramp-and-hold and stretch-to-rupture tests were conducted on a uniaxial tensile apparatus. A nonlinear viscoelastic model with two relaxation coefficients is proposed to fit the ramp-and-hold data. Rupture strain, rupture stress, and elastic modulus of the linear portion of the stress-strain curve are used to characterize the strength properties of the AM. RESULTS: Sample direction has no significant effect on the mechanical properties of the AM. Samples at the ventral region has the maximum rupture strength and elastic modulus, respectively, 2.29 ± 0.99 MPa and 6.26 ± 2.69 MPa. The average of the relaxation coefficient for the fast and slow relaxation phases are 12.8 ± 4.4 s and 37.0 ± 7.7 s, respectively. CONCLUSIONS: AM is a mechanically isotropic and heterogeneous material. The nonlinear viscoelastic model is suitable to model the AM viscoelasticity and potential for other biological tissues.

    Original languageEnglish
    Pages (from-to)603-611
    Number of pages9
    JournalBio-Medical Materials and Engineering
    Volume27
    Issue number6
    DOIs
    Publication statusPublished - 2016

    Fingerprint

    Amnion
    Stress relaxation
    Swine
    Membranes
    Rupture
    Architectural Accessibility
    Nonlinear Dynamics
    Elastic Modulus
    Elastic moduli
    Biological Phenomena
    Mechanical properties
    Viscoelasticity
    Tissue Engineering
    Scaffolds (biology)
    Stress-strain curves
    Tissue engineering
    Scaffolds
    Membrane Potentials
    Tissue

    Keywords

    • Amniotic membrane
    • Mechanical characteristics
    • Mechanical heterogeneousness
    • Nonlinear visceoelastic model
    • Stress relaxation

    ASJC Scopus subject areas

    • Medicine(all)
    • Biomaterials
    • Biomedical Engineering

    Cite this

    Stress relaxation and stress-strain characteristics of porcine amniotic membrane. / Kikuchi, Masato; Feng, Zhonggang; Kosawada, Tadashi; Sato, Daisuke; Nakamura, Takao; Umezu, Mitsuo.

    In: Bio-Medical Materials and Engineering, Vol. 27, No. 6, 2016, p. 603-611.

    Research output: Contribution to journalArticle

    Kikuchi, Masato ; Feng, Zhonggang ; Kosawada, Tadashi ; Sato, Daisuke ; Nakamura, Takao ; Umezu, Mitsuo. / Stress relaxation and stress-strain characteristics of porcine amniotic membrane. In: Bio-Medical Materials and Engineering. 2016 ; Vol. 27, No. 6. pp. 603-611.
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