Abstract
BACKGROUND: Recently, amniotic membrane (AM) as scaffold is accumulating much more attention in tissue engineering. It is well-known that the mechanical properties of the scaffold inevitably affect the biological process of the incorporated cells. OBJECTIVE: This study investigates the stress relaxation and stress-strain characteristics of AM, which have not been sufficiently elucidated before. METHODS: Porcine AMsamples were prepared at four different AMregions and at three different directions. Ramp-and-hold and stretch-to-rupture tests were conducted on a uniaxial tensile apparatus. A nonlinear viscoelastic model with two relaxation coefficients is proposed to fit the ramp-and-hold data. Rupture strain, rupture stress, and elastic modulus of the linear portion of the stress-strain curve are used to characterize the strength properties of the AM. RESULTS: Sample direction has no significant effect on the mechanical properties of the AM. Samples at the ventral region has the maximum rupture strength and elastic modulus, respectively, 2.29 ± 0.99 MPa and 6.26 ± 2.69 MPa. The average of the relaxation coefficient for the fast and slow relaxation phases are 12.8 ± 4.4 s and 37.0 ± 7.7 s, respectively. CONCLUSIONS: AM is a mechanically isotropic and heterogeneous material. The nonlinear viscoelastic model is suitable to model the AM viscoelasticity and potential for other biological tissues.
| Original language | English |
|---|---|
| Pages (from-to) | 603-611 |
| Number of pages | 9 |
| Journal | Bio-Medical Materials and Engineering |
| Volume | 27 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 2016 |
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Keywords
- Amniotic membrane
- Mechanical characteristics
- Mechanical heterogeneousness
- Nonlinear visceoelastic model
- Stress relaxation
ASJC Scopus subject areas
- Medicine(all)
- Biomaterials
- Biomedical Engineering
Cite this
Stress relaxation and stress-strain characteristics of porcine amniotic membrane. / Kikuchi, Masato; Feng, Zhonggang; Kosawada, Tadashi; Sato, Daisuke; Nakamura, Takao; Umezu, Mitsuo.
In: Bio-Medical Materials and Engineering, Vol. 27, No. 6, 2016, p. 603-611.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Stress relaxation and stress-strain characteristics of porcine amniotic membrane
AU - Kikuchi, Masato
AU - Feng, Zhonggang
AU - Kosawada, Tadashi
AU - Sato, Daisuke
AU - Nakamura, Takao
AU - Umezu, Mitsuo
PY - 2016
Y1 - 2016
N2 - BACKGROUND: Recently, amniotic membrane (AM) as scaffold is accumulating much more attention in tissue engineering. It is well-known that the mechanical properties of the scaffold inevitably affect the biological process of the incorporated cells. OBJECTIVE: This study investigates the stress relaxation and stress-strain characteristics of AM, which have not been sufficiently elucidated before. METHODS: Porcine AMsamples were prepared at four different AMregions and at three different directions. Ramp-and-hold and stretch-to-rupture tests were conducted on a uniaxial tensile apparatus. A nonlinear viscoelastic model with two relaxation coefficients is proposed to fit the ramp-and-hold data. Rupture strain, rupture stress, and elastic modulus of the linear portion of the stress-strain curve are used to characterize the strength properties of the AM. RESULTS: Sample direction has no significant effect on the mechanical properties of the AM. Samples at the ventral region has the maximum rupture strength and elastic modulus, respectively, 2.29 ± 0.99 MPa and 6.26 ± 2.69 MPa. The average of the relaxation coefficient for the fast and slow relaxation phases are 12.8 ± 4.4 s and 37.0 ± 7.7 s, respectively. CONCLUSIONS: AM is a mechanically isotropic and heterogeneous material. The nonlinear viscoelastic model is suitable to model the AM viscoelasticity and potential for other biological tissues.
AB - BACKGROUND: Recently, amniotic membrane (AM) as scaffold is accumulating much more attention in tissue engineering. It is well-known that the mechanical properties of the scaffold inevitably affect the biological process of the incorporated cells. OBJECTIVE: This study investigates the stress relaxation and stress-strain characteristics of AM, which have not been sufficiently elucidated before. METHODS: Porcine AMsamples were prepared at four different AMregions and at three different directions. Ramp-and-hold and stretch-to-rupture tests were conducted on a uniaxial tensile apparatus. A nonlinear viscoelastic model with two relaxation coefficients is proposed to fit the ramp-and-hold data. Rupture strain, rupture stress, and elastic modulus of the linear portion of the stress-strain curve are used to characterize the strength properties of the AM. RESULTS: Sample direction has no significant effect on the mechanical properties of the AM. Samples at the ventral region has the maximum rupture strength and elastic modulus, respectively, 2.29 ± 0.99 MPa and 6.26 ± 2.69 MPa. The average of the relaxation coefficient for the fast and slow relaxation phases are 12.8 ± 4.4 s and 37.0 ± 7.7 s, respectively. CONCLUSIONS: AM is a mechanically isotropic and heterogeneous material. The nonlinear viscoelastic model is suitable to model the AM viscoelasticity and potential for other biological tissues.
KW - Amniotic membrane
KW - Mechanical characteristics
KW - Mechanical heterogeneousness
KW - Nonlinear visceoelastic model
KW - Stress relaxation
UR - http://www.scopus.com/inward/record.url?scp=85013459425&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85013459425&partnerID=8YFLogxK
U2 - 10.3233/BME-161612
DO - 10.3233/BME-161612
M3 - Article
C2 - 28234244
AN - SCOPUS:85013459425
VL - 27
SP - 603
EP - 611
JO - Bio-Medical Materials and Engineering
JF - Bio-Medical Materials and Engineering
SN - 0959-2989
IS - 6
ER -