Stromal fibroblasts induce metastatic tumor cell clusters via epithelial–mesenchymal plasticity

Yuko Matsumura, Yasuhiko Ito, Yoshihiro Mezawa, Kaidiliayi Sulidan, Yataro Daigo, Toru Hiraga, Kaoru Mogushi, Nadila Wali, Hiromu Suzuki, Takumi Itoh, Yohei Miyagi, Tomoyuki Yokose, Satoru Shimizu, Atsushi Takano, Yasuhisa Terao, Harumi Saeki, Masayuki Ozawa, Masaaki Abe, Satoru Takeda, Ko OkumuraSonoko Habu, Okio Hino, Kazuyoshi Takeda, Michiaki Hamada, Akira Orimo

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Emerging evidence supports the hypothesis that multicellular tumor clusters invade and seed metastasis. However, whether tumor-associated stroma induces epithelial–mesenchymal plasticity in tumor cell clusters, to promote invasion and metastasis, remains unknown. We demonstrate herein that carcinoma-associated fibroblasts (CAFs) frequently present in tumor stroma drive the formation of tumor cell clusters composed of two distinct cancer cell populations, one in a highly epithelial (E-cadherinhiZEB1lo/neg: Ehi) state and another in a hybrid epithelial/mesenchymal (E-cadherinloZEB1hi: E/M) state. The Ehi cells highly express oncogenic cell–cell adhesion molecules, such as carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5) and CEACAM6 that associate with E-cadherin, resulting in increased tumor cell cluster formation and metastatic seeding. The E/M cells also retain associations with Ehi cells, which follow the E/M cells leading to collective invasion. CAF-produced stromal cell-derived factor 1 and transforming growth factor-β confer the Ehi and E/M states as well as invasive and metastatic traits via Src activation in apposed human breast tumor cells. Taken together, these findings indicate that invasive and metastatic tumor cell clusters are induced by CAFs via epithelial–mesenchymal plasticity.

Original languageEnglish
Article numbere201900425
JournalLife Science Alliance
Volume2
Issue number4
DOIs
Publication statusPublished - 2019 Jan 1

Fingerprint

Fibroblasts
tumor
fibroblasts
Plasticity
plasticity
Tumors
Cells
carcinoma
Neoplasms
metastasis
neoplasms
adhesion
cells
transforming growth factors
Carcinoma
cadherins
Chemokine CXCL12
cell adhesion
breast neoplasms
neoplasm cells

ASJC Scopus subject areas

  • Ecology
  • Biochemistry, Genetics and Molecular Biology (miscellaneous)
  • Plant Science
  • Health, Toxicology and Mutagenesis

Cite this

Matsumura, Y., Ito, Y., Mezawa, Y., Sulidan, K., Daigo, Y., Hiraga, T., ... Orimo, A. (2019). Stromal fibroblasts induce metastatic tumor cell clusters via epithelial–mesenchymal plasticity. Life Science Alliance, 2(4), [e201900425]. https://doi.org/10.26508/lsa.201900425

Stromal fibroblasts induce metastatic tumor cell clusters via epithelial–mesenchymal plasticity. / Matsumura, Yuko; Ito, Yasuhiko; Mezawa, Yoshihiro; Sulidan, Kaidiliayi; Daigo, Yataro; Hiraga, Toru; Mogushi, Kaoru; Wali, Nadila; Suzuki, Hiromu; Itoh, Takumi; Miyagi, Yohei; Yokose, Tomoyuki; Shimizu, Satoru; Takano, Atsushi; Terao, Yasuhisa; Saeki, Harumi; Ozawa, Masayuki; Abe, Masaaki; Takeda, Satoru; Okumura, Ko; Habu, Sonoko; Hino, Okio; Takeda, Kazuyoshi; Hamada, Michiaki; Orimo, Akira.

In: Life Science Alliance, Vol. 2, No. 4, e201900425, 01.01.2019.

Research output: Contribution to journalArticle

Matsumura, Y, Ito, Y, Mezawa, Y, Sulidan, K, Daigo, Y, Hiraga, T, Mogushi, K, Wali, N, Suzuki, H, Itoh, T, Miyagi, Y, Yokose, T, Shimizu, S, Takano, A, Terao, Y, Saeki, H, Ozawa, M, Abe, M, Takeda, S, Okumura, K, Habu, S, Hino, O, Takeda, K, Hamada, M & Orimo, A 2019, 'Stromal fibroblasts induce metastatic tumor cell clusters via epithelial–mesenchymal plasticity', Life Science Alliance, vol. 2, no. 4, e201900425. https://doi.org/10.26508/lsa.201900425
Matsumura, Yuko ; Ito, Yasuhiko ; Mezawa, Yoshihiro ; Sulidan, Kaidiliayi ; Daigo, Yataro ; Hiraga, Toru ; Mogushi, Kaoru ; Wali, Nadila ; Suzuki, Hiromu ; Itoh, Takumi ; Miyagi, Yohei ; Yokose, Tomoyuki ; Shimizu, Satoru ; Takano, Atsushi ; Terao, Yasuhisa ; Saeki, Harumi ; Ozawa, Masayuki ; Abe, Masaaki ; Takeda, Satoru ; Okumura, Ko ; Habu, Sonoko ; Hino, Okio ; Takeda, Kazuyoshi ; Hamada, Michiaki ; Orimo, Akira. / Stromal fibroblasts induce metastatic tumor cell clusters via epithelial–mesenchymal plasticity. In: Life Science Alliance. 2019 ; Vol. 2, No. 4.
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AU - Abe, Masaaki

AU - Takeda, Satoru

AU - Okumura, Ko

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