Structural analysis of the hexasome, lacking one histone H2A/H2B dimer from the conventional nucleosome

Yasuhiro Arimura, Hiroaki Tachiwana, Takashi Oda, Mamoru Sato, Hitoshi Kurumizaka

    Research output: Contribution to journalArticle

    50 Citations (Scopus)

    Abstract

    Genomic DNA is packaged into chromatin in eukaryotes, and the nucleosome is the fundamental unit of chromatin. The canonical nucleosome is the octasome, which is composed of two H2A/H2B dimers and two H3/H4 dimers. During transcription elongation, one of the H2A/H2B dimers is removed from the octasome. The depletion of the H2A/H2B dimer is also suggested to occur during DNA replication and repair. The remaining histone components are believed to maintain a nucleosomal structure called a "hexasome", which is probably important for the regulation of gene expression, DNA replication, and repair in chromatin. However, hexasomes are currently poorly understood, due to the lack of in vivo and in vitro studies. Biochemical and structural studies of hexasomes have been hampered by the difficulty of preparing purified hexasomes. In the present study, we successfully reconstituted hexasomes, using recombinant human histones. A micrococcal nuclease treatment and in vitro reconstitution assays revealed that the hexasome tightly wraps approximately 110 base-pairs of DNA, about 40 base-pairs shorter than the length of the DNA wrapped within the canonical nucleosome. A small-angle X-ray scattering analysis revealed that the global structure of the hexasome is similar to that of the canonical nucleosome. Our studies suggest that octasomes can be converted into hexasomes by the eviction of one of the H2A/H2B dimers, and the release of about 40 base-pairs of DNA, without involving large structural changes in the nucleosome core particle.

    Original languageEnglish
    Pages (from-to)3302-3309
    Number of pages8
    JournalBiochemistry
    Volume51
    Issue number15
    DOIs
    Publication statusPublished - 2012 Apr 17

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    Nucleosomes
    Structural analysis
    Dimers
    Histones
    Base Pairing
    DNA
    Chromatin
    DNA Replication
    DNA Repair
    Repair
    Micrococcal Nuclease
    Gene Expression Regulation
    Eukaryota
    Transcription
    X ray scattering
    Gene expression
    X-Rays
    Elongation
    Assays
    In Vitro Techniques

    ASJC Scopus subject areas

    • Biochemistry

    Cite this

    Structural analysis of the hexasome, lacking one histone H2A/H2B dimer from the conventional nucleosome. / Arimura, Yasuhiro; Tachiwana, Hiroaki; Oda, Takashi; Sato, Mamoru; Kurumizaka, Hitoshi.

    In: Biochemistry, Vol. 51, No. 15, 17.04.2012, p. 3302-3309.

    Research output: Contribution to journalArticle

    Arimura, Y, Tachiwana, H, Oda, T, Sato, M & Kurumizaka, H 2012, 'Structural analysis of the hexasome, lacking one histone H2A/H2B dimer from the conventional nucleosome', Biochemistry, vol. 51, no. 15, pp. 3302-3309. https://doi.org/10.1021/bi300129b
    Arimura, Yasuhiro ; Tachiwana, Hiroaki ; Oda, Takashi ; Sato, Mamoru ; Kurumizaka, Hitoshi. / Structural analysis of the hexasome, lacking one histone H2A/H2B dimer from the conventional nucleosome. In: Biochemistry. 2012 ; Vol. 51, No. 15. pp. 3302-3309.
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