Structural and dynamical characteristics of tropomyosin epitopes as the major allergens in shrimp

Hideo Ozawa, Koji Umezawa, Mitsunori Takano, Shoichiro Ishizaki, Shugo Watabe, Yoshihiro Ochiai

    Research output: Contribution to journalArticle

    5 Citations (Scopus)

    Abstract

    Ingestion of marine invertebrates often causes food allergy, where the major allergens have been reported to be derived from tropomyosin (TM). Intact or the digestive fragments of food allergens generally show resistance to digestion, which is usually attributable to the structural stability (or rigidity). The difference in the structural and dynamical characteristics between the epitope and the non-epitope regions in TM has not yet been well understood. In the present study, molecular dynamics simulation was performed at constant pHs for shrimp TM. By analyzing the main-chain dihedral angle fluctuations and local α-helix contents, we found that the epitope regions are more stable than the non-epitope counterparts, providing a possible physical reason for the resistance to digestion in the epitopes regions. The difference of the structural stability between the epitope and the non-epitope regions was largest at low pHs, even though pH dependence of the structural stability in itself was not significant in both regions. The lower content of the Ala cluster in the epitope region is considered to cause the higher stability of the epitope region.

    Original languageEnglish
    Pages (from-to)119-124
    Number of pages6
    JournalBiochemical and Biophysical Research Communications
    Volume498
    Issue number1
    DOIs
    Publication statusPublished - 2018 Mar 25

    Fingerprint

    Tropomyosin
    Allergens
    Epitopes
    Digestion
    Allergies
    Food Hypersensitivity
    Dihedral angle
    Invertebrates
    Molecular Dynamics Simulation
    Rigidity
    Molecular dynamics
    Eating
    Food
    Computer simulation

    Keywords

    • Allergen
    • Coiled-coil
    • Epitope
    • Molecular dynamics simulation
    • Shrimp
    • Tropomyosin

    ASJC Scopus subject areas

    • Biophysics
    • Biochemistry
    • Molecular Biology
    • Cell Biology

    Cite this

    Structural and dynamical characteristics of tropomyosin epitopes as the major allergens in shrimp. / Ozawa, Hideo; Umezawa, Koji; Takano, Mitsunori; Ishizaki, Shoichiro; Watabe, Shugo; Ochiai, Yoshihiro.

    In: Biochemical and Biophysical Research Communications, Vol. 498, No. 1, 25.03.2018, p. 119-124.

    Research output: Contribution to journalArticle

    Ozawa, Hideo ; Umezawa, Koji ; Takano, Mitsunori ; Ishizaki, Shoichiro ; Watabe, Shugo ; Ochiai, Yoshihiro. / Structural and dynamical characteristics of tropomyosin epitopes as the major allergens in shrimp. In: Biochemical and Biophysical Research Communications. 2018 ; Vol. 498, No. 1. pp. 119-124.
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