Structural and functional characteristics of S-like ribonucleases from carnivorous plants

Emi Nishimura, Shinya Jumyo, Naoki Arai, Kensuke Kanna, Marina Kume, Jun ichi Nishikawa, Jun ichi Tanase, Takashi Ohyama

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    11 Citations (Scopus)

    Abstract

    Although the S-like ribonucleases (RNases) share sequence homology with the S-RNases involved in the self-incompatibility mechanism in plants, they are not associated with this mechanism. They usually function in stress responses in non-carnivorous plants and in carnivory in carnivorous plants. In this study, we clarified the structures of the S-like RNases of Aldrovanda vesiculosa, Nepenthes bicalcarata and Sarracenia leucophylla, and compared them with those of other plants. At ten positions, amino acid residues are conserved or almost conserved only for carnivorous plants (six in total). In contrast, two positions are specific to non-carnivorous plants. A phylogenetic analysis revealed that the S-like RNases of the carnivorous plants form a group beyond the phylogenetic relationships of the plants. We also prepared and characterized recombinant S-like RNases of Dionaea muscipula, Cephalotus follicularis, A. vesiculosa, N. bicalcarata and S. leucophylla, and RNS1 of Arabidopsis thaliana. The recombinant carnivorous plant enzymes showed optimum activities at about pH 4.0. Generally, poly(C) was digested less efficiently than poly(A), poly(I) and poly(U). The kinetic parameters of the recombinant D. muscipula enzyme (DM-I) and A. thaliana enzyme RNS1 were similar. The k cat/Km of recombinant RNS1 was the highest among the enzymes, followed closely by that of recombinant DM-I. On the other hand, the kcat/Km of the recombinant S. leucophylla enzyme was the lowest, and was ~1/30 of that for recombinant RNS1. The magnitudes of the kcat/Km values or kcat values for carnivorous plant S-like RNases seem to correlate negatively with the dependency on symbionts for prey digestion.

    Original languageEnglish
    Pages (from-to)147-159
    Number of pages13
    JournalPlanta
    Volume240
    Issue number1
    DOIs
    Publication statusPublished - 2014

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    Keywords

    • Droseraceae
    • Enzyme kinetics
    • Nepenthaceae
    • Phylogenetic tree
    • Protein structure
    • Recombinant protein

    ASJC Scopus subject areas

    • Plant Science
    • Genetics

    Cite this

    Nishimura, E., Jumyo, S., Arai, N., Kanna, K., Kume, M., Nishikawa, J. I., Tanase, J. I., & Ohyama, T. (2014). Structural and functional characteristics of S-like ribonucleases from carnivorous plants. Planta, 240(1), 147-159. https://doi.org/10.1007/s00425-014-2072-8