Structural basis for the DNA-binding activity of the bacterial Β-propeller protein YncE

Wataru Kagawa, Tomohiko Sagawa, Hironori Niki, Hitoshi Kurumizaka

    Research output: Contribution to journalArticle

    6 Citations (Scopus)

    Abstract

    Β-Propellers are widely utilized in nature as recognition modules. The well conserved Β-propeller fold exhibits a high degree of functional diversity, which is probably accomplished through variations in the surface properties of the proteins. Little is known about the interactions between Β-propeller proteins and nucleic acids. In the present study, it has been found that the bacterial Β-propeller protein YncE binds to DNA. Crystal structures of YncE in the free form and complexed with DNA revealed that the surface region of YncE corresponding to the canonical substrate-binding site forms essential contacts with DNA. A single DNA base within a single-stranded DNA region is trapped in the hydrophobic pocket located within the central channel of the Β-propeller protein. These data provide physical evidence for the DNA-binding ability of the previously uncharacterized YncE and also suggest that the canonical substrate-binding site may be commonly adapted to facilitate nucleic acid binding in a subset of Β-propeller proteins.

    Original languageEnglish
    Pages (from-to)1045-1053
    Number of pages9
    JournalActa Crystallographica Section D: Biological Crystallography
    Volume67
    Issue number12
    DOIs
    Publication statusPublished - 2011 Dec

    Fingerprint

    Bacterial Proteins
    DNA
    Nucleic Acids
    Binding Sites
    Proteins
    Surface Properties
    Single-Stranded DNA
    Membrane Proteins

    Keywords

    • Β-propellers
    • DNA
    • YncE

    ASJC Scopus subject areas

    • Structural Biology

    Cite this

    Structural basis for the DNA-binding activity of the bacterial Β-propeller protein YncE. / Kagawa, Wataru; Sagawa, Tomohiko; Niki, Hironori; Kurumizaka, Hitoshi.

    In: Acta Crystallographica Section D: Biological Crystallography, Vol. 67, No. 12, 12.2011, p. 1045-1053.

    Research output: Contribution to journalArticle

    Kagawa, Wataru ; Sagawa, Tomohiko ; Niki, Hironori ; Kurumizaka, Hitoshi. / Structural basis for the DNA-binding activity of the bacterial Β-propeller protein YncE. In: Acta Crystallographica Section D: Biological Crystallography. 2011 ; Vol. 67, No. 12. pp. 1045-1053.
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