Structural basis for the substrate recognition and catalysis of peptidyl-tRNA hydrolase

Kosuke Ito, Ryo Murakami, Masahiro Mochizuki, Hao Qi, Yoshihiro Shimizu, Kin Ichiro Miura, Takuya Ueda, Toshio Uchiumi

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Peptidyl-tRNA hydrolase (Pth) cleaves the ester bond between the peptide and the tRNA of peptidyl-tRNA molecules, which are produced by aborted translation, to recycle tRNA for further rounds of protein synthesis. Pth is ubiquitous in nature, and its enzymatic activity is essential for bacterial viability. We have determined the crystal structure of Escherichia coli Pth in complex with the tRNA CCA-acceptor-TΨC domain, the enzyme-binding region of the tRNA moiety of the substrate, at 2.4 Å resolution. In combination with site-directed mutagenesis studies, the structure identified the amino acid residues involved in tRNA recognition. The structure also revealed that Pth interacts with the tRNA moiety through the backbone phosphates and riboses, and no base-specific interactions were observed, except for the interaction with the highly conserved base G53. This feature enables Pth to accept the diverse sequences of the elongator-tRNAs as substrate components. Furthermore, we propose an authentic Pth:peptidyl-tRNA complex model and a detailed mechanism for the hydrolysis reaction, based on the present crystal structure and the previous studies' results.

Original languageEnglish
Pages (from-to)10521-10531
Number of pages11
JournalNucleic acids research
Volume40
Issue number20
DOIs
Publication statusPublished - 2012 Nov 1
Externally publishedYes

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Transfer RNA
Catalysis
Microbial Viability
Ribose
Site-Directed Mutagenesis
aminoacyl-tRNA hydrolase
Esters
Hydrolysis
Phosphates
Escherichia coli
Amino Acids
Peptides
Enzymes
Proteins

ASJC Scopus subject areas

  • Genetics

Cite this

Ito, K., Murakami, R., Mochizuki, M., Qi, H., Shimizu, Y., Miura, K. I., ... Uchiumi, T. (2012). Structural basis for the substrate recognition and catalysis of peptidyl-tRNA hydrolase. Nucleic acids research, 40(20), 10521-10531. https://doi.org/10.1093/nar/gks790

Structural basis for the substrate recognition and catalysis of peptidyl-tRNA hydrolase. / Ito, Kosuke; Murakami, Ryo; Mochizuki, Masahiro; Qi, Hao; Shimizu, Yoshihiro; Miura, Kin Ichiro; Ueda, Takuya; Uchiumi, Toshio.

In: Nucleic acids research, Vol. 40, No. 20, 01.11.2012, p. 10521-10531.

Research output: Contribution to journalArticle

Ito, K, Murakami, R, Mochizuki, M, Qi, H, Shimizu, Y, Miura, KI, Ueda, T & Uchiumi, T 2012, 'Structural basis for the substrate recognition and catalysis of peptidyl-tRNA hydrolase', Nucleic acids research, vol. 40, no. 20, pp. 10521-10531. https://doi.org/10.1093/nar/gks790
Ito K, Murakami R, Mochizuki M, Qi H, Shimizu Y, Miura KI et al. Structural basis for the substrate recognition and catalysis of peptidyl-tRNA hydrolase. Nucleic acids research. 2012 Nov 1;40(20):10521-10531. https://doi.org/10.1093/nar/gks790
Ito, Kosuke ; Murakami, Ryo ; Mochizuki, Masahiro ; Qi, Hao ; Shimizu, Yoshihiro ; Miura, Kin Ichiro ; Ueda, Takuya ; Uchiumi, Toshio. / Structural basis for the substrate recognition and catalysis of peptidyl-tRNA hydrolase. In: Nucleic acids research. 2012 ; Vol. 40, No. 20. pp. 10521-10531.
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